In vivo optical virtual biopsy of human oral cavity with harmonic generation microscopy

M. R. Tsai, S. Y. Chen, Dar-Bin Shieh, P. J. Lou, C. K. Sun

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Oral cancer ranked number four in both cancer incident and mortality in Taiwanese male population. Early disease diagnosis and staging is essential for its clinical success. However, most patients were diagnosed in their late disease stage as ideal prescreening procedures are yet to be developed especially when dealing with a large surface of precancerous lesions. Therefore, how to detect and confirm the diagnosis of these early stage lesions are of significant clinical value. Harmonic generation process naturally occurred in biological molecules and requires no energy deposition to the target molecule. Thus harmonic generation microscopy (HGM) could potentially serve as a noninvasive tool for screening of human oral mucosal diseases. The in vivo optical biopsy of human oral cavity with HGM could be achieved with high spatial resolution to resolve dynamic physiological process in the oral mucosal tissue with equal or superior quality but devoid of complicated physical biopsy procedures. The second harmonic generation (SHG) provide significant image contrast for biomolecules with repetitive structures such as the collagen fibers in the lamina propria and the mitotic spindles in dividing cells. The cell morphology in the epithelial layer, blood vessels and blood cells flow through the capillaries can be revealed by third harmonic generation (THG) signals. Tissue transparent technology was used to increase the optical penetration of the tissue. In conclusion, this report demonstrates the first in vivo optical virtual biopsy of human oral mucosa using HGM and revealed a promising future for its clinical application for noninvasive in vivo diseases diagnosis.

Original languageEnglish
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences X
DOIs
Publication statusPublished - 2010 May 7
EventMultiphoton Microscopy in the Biomedical Sciences X - San Francisco, CA, United States
Duration: 2010 Jan 242010 Jan 26

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7569
ISSN (Print)1605-7422

Other

OtherMultiphoton Microscopy in the Biomedical Sciences X
CountryUnited States
CitySan Francisco, CA
Period10-01-2410-01-26

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All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

Cite this

Tsai, M. R., Chen, S. Y., Shieh, D-B., Lou, P. J., & Sun, C. K. (2010). In vivo optical virtual biopsy of human oral cavity with harmonic generation microscopy. In Multiphoton Microscopy in the Biomedical Sciences X [75691Q] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7569). https://doi.org/10.1117/12.842223