In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods

Chen Wei Wei, Chao Kang Liao, Ying Yi Chen, Churng Ren Chris Wang, Ann Ann Ding, Dar-Bin Shieh, Pai Chi Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

In this study, photoacoustic imaging is utilized to probe information from oncogene surface molecules of cancer cell with the aid of specific targeting. The ultimate goal is to provide prediction of clinical outcome and treatment response of anti-cancer drugs. Different from single targeting in most research, we accomplished multiple targeting to obtain a molecular profile potentially representing tumor characteristics or to locate the heterogeneous population in one lesion. By conjugating different antibodies to gold nanorods corresponding to different peak absorption bands, multiple targeting and simultaneous detection with photoacoustic imaging can be achieved with laser irradiation at the respective peak optical absorption wavelength. Her2 and EGFR were chosen as our primary target molecules. The targeting complex was evaluated in two types of oral cancer cells, OECM1 and Cal27. The OECM1 cell line overexpresses Her2 but has low expression of EGFR, while Cal27 cell line expresses both antibodies. Also, the targeting efficacy to OECM1 can be further improved by using mixed nanoprobes. The cancer cells were induced on the back of the mice by subcutaneous injection. The captured images show that both cancer cells exhibit a higher photoacoustic response (maximum 3 dB) than control groups with specific targeting, thus demonstrating the feasibility of multiple selective targeting with bioconjugated gold nanorods. Images of multiple targeting with mixed nanoprobes of OECM1 cells also reveal further enhancement of targeting (4 dB). The results showed potential of in vivo photoacoustic molecular imaging, providing a better guidance for diagnosis and treatment of cancer.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics
DOIs
Publication statusPublished - 2008 Apr 21
Event9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008 - San Jose, CA, United States
Duration: 2008 Jan 202008 Jan 23

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6856
ISSN (Print)1605-7422

Other

Other9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008
CountryUnited States
CitySan Jose, CA
Period08-01-2008-01-23

Fingerprint

Photoacoustic effect
Nanorods
Gold
Cells
Imaging techniques
Nanoprobes
Antibodies
Molecular imaging
Molecules
Laser beam effects
Light absorption
Tumors
Absorption spectra
Wavelength

All Science Journal Classification (ASJC) codes

  • Engineering(all)

Cite this

Wei, C. W., Liao, C. K., Chen, Y. Y., Wang, C. R. C., Ding, A. A., Shieh, D-B., & Li, P. C. (2008). In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods. In Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics [68560J] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6856). https://doi.org/10.1117/12.764454
Wei, Chen Wei ; Liao, Chao Kang ; Chen, Ying Yi ; Wang, Churng Ren Chris ; Ding, Ann Ann ; Shieh, Dar-Bin ; Li, Pai Chi. / In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods. Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. 2008. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).
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title = "In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods",
abstract = "In this study, photoacoustic imaging is utilized to probe information from oncogene surface molecules of cancer cell with the aid of specific targeting. The ultimate goal is to provide prediction of clinical outcome and treatment response of anti-cancer drugs. Different from single targeting in most research, we accomplished multiple targeting to obtain a molecular profile potentially representing tumor characteristics or to locate the heterogeneous population in one lesion. By conjugating different antibodies to gold nanorods corresponding to different peak absorption bands, multiple targeting and simultaneous detection with photoacoustic imaging can be achieved with laser irradiation at the respective peak optical absorption wavelength. Her2 and EGFR were chosen as our primary target molecules. The targeting complex was evaluated in two types of oral cancer cells, OECM1 and Cal27. The OECM1 cell line overexpresses Her2 but has low expression of EGFR, while Cal27 cell line expresses both antibodies. Also, the targeting efficacy to OECM1 can be further improved by using mixed nanoprobes. The cancer cells were induced on the back of the mice by subcutaneous injection. The captured images show that both cancer cells exhibit a higher photoacoustic response (maximum 3 dB) than control groups with specific targeting, thus demonstrating the feasibility of multiple selective targeting with bioconjugated gold nanorods. Images of multiple targeting with mixed nanoprobes of OECM1 cells also reveal further enhancement of targeting (4 dB). The results showed potential of in vivo photoacoustic molecular imaging, providing a better guidance for diagnosis and treatment of cancer.",
author = "Wei, {Chen Wei} and Liao, {Chao Kang} and Chen, {Ying Yi} and Wang, {Churng Ren Chris} and Ding, {Ann Ann} and Dar-Bin Shieh and Li, {Pai Chi}",
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Wei, CW, Liao, CK, Chen, YY, Wang, CRC, Ding, AA, Shieh, D-B & Li, PC 2008, In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods. in Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics., 68560J, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6856, 9th Conference on Photons Plus Ultrasound: Imaging and Sensing 2008, San Jose, CA, United States, 08-01-20. https://doi.org/10.1117/12.764454

In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods. / Wei, Chen Wei; Liao, Chao Kang; Chen, Ying Yi; Wang, Churng Ren Chris; Ding, Ann Ann; Shieh, Dar-Bin; Li, Pai Chi.

Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. 2008. 68560J (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6856).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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T1 - In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods

AU - Wei, Chen Wei

AU - Liao, Chao Kang

AU - Chen, Ying Yi

AU - Wang, Churng Ren Chris

AU - Ding, Ann Ann

AU - Shieh, Dar-Bin

AU - Li, Pai Chi

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N2 - In this study, photoacoustic imaging is utilized to probe information from oncogene surface molecules of cancer cell with the aid of specific targeting. The ultimate goal is to provide prediction of clinical outcome and treatment response of anti-cancer drugs. Different from single targeting in most research, we accomplished multiple targeting to obtain a molecular profile potentially representing tumor characteristics or to locate the heterogeneous population in one lesion. By conjugating different antibodies to gold nanorods corresponding to different peak absorption bands, multiple targeting and simultaneous detection with photoacoustic imaging can be achieved with laser irradiation at the respective peak optical absorption wavelength. Her2 and EGFR were chosen as our primary target molecules. The targeting complex was evaluated in two types of oral cancer cells, OECM1 and Cal27. The OECM1 cell line overexpresses Her2 but has low expression of EGFR, while Cal27 cell line expresses both antibodies. Also, the targeting efficacy to OECM1 can be further improved by using mixed nanoprobes. The cancer cells were induced on the back of the mice by subcutaneous injection. The captured images show that both cancer cells exhibit a higher photoacoustic response (maximum 3 dB) than control groups with specific targeting, thus demonstrating the feasibility of multiple selective targeting with bioconjugated gold nanorods. Images of multiple targeting with mixed nanoprobes of OECM1 cells also reveal further enhancement of targeting (4 dB). The results showed potential of in vivo photoacoustic molecular imaging, providing a better guidance for diagnosis and treatment of cancer.

AB - In this study, photoacoustic imaging is utilized to probe information from oncogene surface molecules of cancer cell with the aid of specific targeting. The ultimate goal is to provide prediction of clinical outcome and treatment response of anti-cancer drugs. Different from single targeting in most research, we accomplished multiple targeting to obtain a molecular profile potentially representing tumor characteristics or to locate the heterogeneous population in one lesion. By conjugating different antibodies to gold nanorods corresponding to different peak absorption bands, multiple targeting and simultaneous detection with photoacoustic imaging can be achieved with laser irradiation at the respective peak optical absorption wavelength. Her2 and EGFR were chosen as our primary target molecules. The targeting complex was evaluated in two types of oral cancer cells, OECM1 and Cal27. The OECM1 cell line overexpresses Her2 but has low expression of EGFR, while Cal27 cell line expresses both antibodies. Also, the targeting efficacy to OECM1 can be further improved by using mixed nanoprobes. The cancer cells were induced on the back of the mice by subcutaneous injection. The captured images show that both cancer cells exhibit a higher photoacoustic response (maximum 3 dB) than control groups with specific targeting, thus demonstrating the feasibility of multiple selective targeting with bioconjugated gold nanorods. Images of multiple targeting with mixed nanoprobes of OECM1 cells also reveal further enhancement of targeting (4 dB). The results showed potential of in vivo photoacoustic molecular imaging, providing a better guidance for diagnosis and treatment of cancer.

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Wei CW, Liao CK, Chen YY, Wang CRC, Ding AA, Shieh D-B et al. In vivo photoacoustic imaging with multiple selective targeting using bioconjugated gold nanorods. In Photons Plus Ultrasound: Imaging and Sensing 2008: The Ninth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics. 2008. 68560J. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.764454