Increased expression of argininosuccinate synthetase protein predicts poor prognosis in human gastric cancer

Yan Shen Shan, Hui Ping Hsu, Ming Derg Lai, Meng Chi Yen, Yi Pey Luo, Yi Ling Chen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Aberrant expression of argininosuccinate synthetase (ASS1, also known as ASS) has been found in cancer cells and is involved in the carcinogenesis of gastric cancer. The aim of the present study was to investigate the level of ASS expression in human gastric cancer and to determine the possible correlations between ASS expression and clinicopathological findings. Immunohistochemistry was performed on paraffin-embedded tissues to determine whether ASS was expressed in 11 of 11 specimens from patients with gastric cancer. The protein was localized primarily to the cytoplasm of cancer cells and normal epithelium. In the Oncomine cancer microarray database, expression of the ASS gene was significantly increased in gastric cancer tissues. To investigate the clinicopathological and prognostic roles of ASS expression, we performed western blot analysis of 35 matched specimens of gastric adenocarcinomas and normal tissue obtained from patients treated at the National Cheng Kung University Hospital. The ratio of relative ASS expression (expressed as the ASS/β-actin ratio) in tumor tissues to that in normal tissues was correlated with large tumor size (P=0.007) and with the tumor, node, metastasis (TNM) stage of the American Joint Committee on Cancer staging system (P=0.031). Patients whose cancer had increased the relative expression of ASS were positive for perineural invasion and had poor recurrence free survival. In summary, ASS expression in gastric cancer was associated with a poor prognosis. Further study of mechanisms to silence the ASS gene or decrease the enzymatic activity of ASS protein has the potential to provide new treatments for patients with gastric cancer.

Original languageEnglish
Pages (from-to)49-57
Number of pages9
JournalOncology Reports
Volume33
Issue number1
DOIs
Publication statusPublished - 2015 Jan 1

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Argininosuccinate Synthase
Stomach Neoplasms
Neoplasms
Proteins
Neoplasm Staging
Paraffin
Actins
Stomach
Carcinogenesis
Cytoplasm
Adenocarcinoma
Epithelium
Western Blotting
Immunohistochemistry
Databases
Neoplasm Metastasis
Gene Expression
Recurrence
Survival

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

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title = "Increased expression of argininosuccinate synthetase protein predicts poor prognosis in human gastric cancer",
abstract = "Aberrant expression of argininosuccinate synthetase (ASS1, also known as ASS) has been found in cancer cells and is involved in the carcinogenesis of gastric cancer. The aim of the present study was to investigate the level of ASS expression in human gastric cancer and to determine the possible correlations between ASS expression and clinicopathological findings. Immunohistochemistry was performed on paraffin-embedded tissues to determine whether ASS was expressed in 11 of 11 specimens from patients with gastric cancer. The protein was localized primarily to the cytoplasm of cancer cells and normal epithelium. In the Oncomine cancer microarray database, expression of the ASS gene was significantly increased in gastric cancer tissues. To investigate the clinicopathological and prognostic roles of ASS expression, we performed western blot analysis of 35 matched specimens of gastric adenocarcinomas and normal tissue obtained from patients treated at the National Cheng Kung University Hospital. The ratio of relative ASS expression (expressed as the ASS/β-actin ratio) in tumor tissues to that in normal tissues was correlated with large tumor size (P=0.007) and with the tumor, node, metastasis (TNM) stage of the American Joint Committee on Cancer staging system (P=0.031). Patients whose cancer had increased the relative expression of ASS were positive for perineural invasion and had poor recurrence free survival. In summary, ASS expression in gastric cancer was associated with a poor prognosis. Further study of mechanisms to silence the ASS gene or decrease the enzymatic activity of ASS protein has the potential to provide new treatments for patients with gastric cancer.",
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Increased expression of argininosuccinate synthetase protein predicts poor prognosis in human gastric cancer. / Shan, Yan Shen; Hsu, Hui Ping; Lai, Ming Derg; Yen, Meng Chi; Luo, Yi Pey; Chen, Yi Ling.

In: Oncology Reports, Vol. 33, No. 1, 01.01.2015, p. 49-57.

Research output: Contribution to journalArticle

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AU - Hsu, Hui Ping

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AU - Chen, Yi Ling

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