Indispensability of the Escherichia coli Carbonic Anhydrases YadF and CynT in Cell Proliferation at a Low CO2 Partial Pressure

Masayuki Hashimoto; Jun Ichi Kato

doi:10.1271/bbb.67.919

Indispensability of the Escherichia coli Carbonic Anhydrases YadF and CynT in Cell Proliferation at a Low CO₂ Partial Pressure

Masayuki Hashimoto, Jun Ichi Kato

Institute of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

32 Citations (Scopus)

Abstract

We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli. However, mutant strains lacking both of them grew at high CO₂ concentrations (5%), where non-enzymatic mechanisms generate HCO₃⁻. This suggests that these carbonic anhydrases are essential because they maintain HCO₃⁻ levels at ambient CO₂ concentrations.

Original language	English
Pages (from-to)	919-922
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	67
Issue number	4
DOIs	https://doi.org/10.1271/bbb.67.919
Publication status	Published - 2003 Jan 1

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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Indispensability of the Escherichia coli Carbonic Anhydrases YadF and CynT in Cell Proliferation at a Low CO₂ Partial Pressure

Abstract

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