Indispensability of the Escherichia coli Carbonic Anhydrases YadF and CynT in Cell Proliferation at a Low CO2 Partial Pressure

Masayuki Hashimoto; Jun Ichi Kato

doi:10.1271/bbb.67.919

Indispensability of the Escherichia coli Carbonic Anhydrases YadF and CynT in Cell Proliferation at a Low CO₂ Partial Pressure

Masayuki Hashimoto, Jun Ichi Kato

Institute of Molecular Medicine

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli. However, mutant strains lacking both of them grew at high CO₂ concentrations (5%), where non-enzymatic mechanisms generate HCO₃⁻. This suggests that these carbonic anhydrases are essential because they maintain HCO₃⁻ levels at ambient CO₂ concentrations.

Original language	English
Pages (from-to)	919-922
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	67
Issue number	4
DOIs	https://doi.org/10.1271/bbb.67.919
Publication status	Published - 2003 Jan 1

All Science Journal Classification (ASJC) codes

Biotechnology
Analytical Chemistry
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology
Organic Chemistry

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abstract = "We found that a carbonic anhydrase, YadF, is essential for cell growth in the absence of another carbonic anhydrase, CynT, in Escherichia coli. However, mutant strains lacking both of them grew at high CO2 concentrations (5%), where non-enzymatic mechanisms generate HCO3−. This suggests that these carbonic anhydrases are essential because they maintain HCO3− levels at ambient CO2 concentrations.",

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