Induced interleukin-8 expression in gliomas by tumor-associated macrophages

Tse-Ming Hong, Lee Jene Teng, Chia Tung Shun, Mei Chen Peng, Jui Chang Tsai

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The tumor microenvironment affects tumor initiation, progression, and metastasis. However, it is still not clear how stromal cells interact with the tumor cells. By using a cytokine array immunoblot assay, we showed that interleukin (IL)-8, IL-6, and RANTES (regulated upon activation normal T-cell expressed and secreted) proteins were up-regulated in GBM8401 glioma cells after coculture with human THP-1-derived macrophages. IL-8 is a chemokine with leukocyte chemotactic, tumorigenic, and proangiogenic properties. To evaluate the correlation of IL-8 expression with tumor-associated macrophages and angiogenesis, 43 glioma specimens were studied. The results showed that the IL-8 mRNA expression and microvessel count in glioma surgical specimens correlated positively with the density of tumor-associated macrophages. We further showed that IL-8 mRNA expression in GBM8401 cells increased dramatically, by 2 8-2 10-fold, after being cocultured with macrophages. This increase could also be induced by macrophage-conditioned medium, tumor necrosis factor-α, IL-1α, and IL-1β, and could be suppressed by anti-inflammatory agents including pyrrolidine dithiocarbamate, pentoxifylline, or dexamethasone. These findings imply that macrophage infiltration may be the common feature shared by cancer and inflammation, and macrophages could play a role in promoting glioma growth and angiogenesis by inducing IL-8 expression in glioma cells via inflammatory stimuli or the nuclear factor kappa B pathway.

Original languageEnglish
Pages (from-to)289-301
Number of pages13
JournalJournal of Neuro-Oncology
Volume93
Issue number3
DOIs
Publication statusPublished - 2009 Jan 22

Fingerprint

Interleukin-8
Glioma
Macrophages
Neoplasms
Interleukin-1
Pentoxifylline
Messenger RNA
Tumor Microenvironment
NF-kappa B
Stromal Cells
Conditioned Culture Medium
Coculture Techniques
Microvessels
Chemokines
Dexamethasone
Interleukin-6
Leukocytes
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Cytokines

All Science Journal Classification (ASJC) codes

  • Clinical Neurology
  • Cancer Research
  • Oncology
  • Neurology

Cite this

Hong, Tse-Ming ; Teng, Lee Jene ; Shun, Chia Tung ; Peng, Mei Chen ; Tsai, Jui Chang. / Induced interleukin-8 expression in gliomas by tumor-associated macrophages. In: Journal of Neuro-Oncology. 2009 ; Vol. 93, No. 3. pp. 289-301.
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Induced interleukin-8 expression in gliomas by tumor-associated macrophages. / Hong, Tse-Ming; Teng, Lee Jene; Shun, Chia Tung; Peng, Mei Chen; Tsai, Jui Chang.

In: Journal of Neuro-Oncology, Vol. 93, No. 3, 22.01.2009, p. 289-301.

Research output: Contribution to journalArticle

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