Induced pluripotent stem cell (iPSC) line MLi005-A derived from a patient with dominant dystrophic epidermolysis bullosa (DDEB)

Sandra Ropret, Preeti Khurana, Tara Fabčič, Janina Cvetkovska, Ana Trobec, Špela Zemljič Jokhadar, Dusko Ilic, John A. McGrath, Christina Guttmann-Gruber, Mirjana Liovic

Research output: Contribution to journalArticlepeer-review

Abstract

We have generated MLi005-A, a new induced pluripotent stem cell (iPSC) line derived from skin fibroblasts of a male patient with dominant dystrophic epidermolysis bullosa (DDEB). This iPSC line may be used as a model system for studies on skin integrity, the extracellular matrix and skin barrier function. The characterization of the MLi005-A cell line consisted of molecular karyotyping, next-generation sequencing of the COL7A1 alleles, pluripotency and differentiation potentials testing by immunofluorescence of associated markers in vitro. The MLi-005A line has been also tested for ability to differentiate into fibroblasts and keratinocytes and markers associated with these cell types.

Original languageEnglish
Article number103306
JournalStem Cell Research
Volume75
DOIs
Publication statusPublished - 2024 Mar

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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