Inducible β-lactam resistance in Aeromonas hydrophila

Therapeutic challenge for antimicrobial therapy

Wen-Chien Ko, Hsiu Mei Wu, Tsung Chain Chang, Jing Jou Yan, Jiunn Jong Wu

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Despite the abundant amount of knowledge about inducible chromosomally mediated β-lactamases among Aeromonas species, extended-spectrum β-lactam- resistant A. hydrophila strains selected in clinical practice were rarely reported. In the present study, two strains of A. hydrophila, A136 and A139, with markedly different susceptibilities to extended-spectrum cephalosporins were isolated from blood and the tip segment of an arterial catheter of a burn patient. Another strain (A136m) was selected in vitro by culturing A136 in a subinhibitory concentration of cefotaxime, the β-lactam agent administered for the treatment of Aeromonas bacteremia in this patient. Typing studies by arbitrarily primed PCR and pulsed-field gel electrophoresis indicated a clonal relationship among strains A136, A136m, and A139. These strains were identified to be of DNA hybridization group 1. Wild-type strain A136 was resistant only to ampicillin and cephamycins, but A136m and A139 were highly resistant to the expanded- and broad-spectrum cephalosporins. The presence of increased β-lactamase activity in A139 suggests that A139 is a derepressed mutant which overexpresses β-lactamases. These results call attention to the use of β-lactam agents for the treatment of invasive Aeromonas infections.

Original languageEnglish
Pages (from-to)3188-3192
Number of pages5
JournalJournal of Clinical Microbiology
Volume36
Issue number11
Publication statusPublished - 1998 Nov 1

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Aeromonas hydrophila
Aeromonas
Lactams
Cephalosporins
Cephamycins
Cefotaxime
Pulsed Field Gel Electrophoresis
Ampicillin
Bacteremia
Therapeutics
Catheters
Polymerase Chain Reaction
DNA
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Ko, Wen-Chien ; Wu, Hsiu Mei ; Chang, Tsung Chain ; Yan, Jing Jou ; Wu, Jiunn Jong. / Inducible β-lactam resistance in Aeromonas hydrophila : Therapeutic challenge for antimicrobial therapy. In: Journal of Clinical Microbiology. 1998 ; Vol. 36, No. 11. pp. 3188-3192.
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Inducible β-lactam resistance in Aeromonas hydrophila : Therapeutic challenge for antimicrobial therapy. / Ko, Wen-Chien; Wu, Hsiu Mei; Chang, Tsung Chain; Yan, Jing Jou; Wu, Jiunn Jong.

In: Journal of Clinical Microbiology, Vol. 36, No. 11, 01.11.1998, p. 3188-3192.

Research output: Contribution to journalArticle

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