Induction of 12-lipoxygenase expression by transforming growth factor-α in human epidermoid carcinoma A431 cells

Lei Chin Chen, Ben Kuen Chen, Yi Wen Liu, Wen Chang Chang

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13 Citations (Scopus)

Abstract

Transforming growth factor-α (TGF-α) increased the expression of 12-lipoxygenase activity in a time-dependent manner in human epidermoid carcinoma A431 cells. The increase of 12-lipoxygenase activity was accompanied by an increase in 12-lipoxygenase mRNA. The effect of TGF-α on the promoter activation of 12-lipoxygenase gene was analyzed by using the luciferase fusion vectors. A dose-dependent effect of TGF-α on the reporter activity was observed, which paralleled with its effect on enzyme activity. Transient transfection with a series of 5'-deleted constructs showed that the 5'-flanking region spanning from -224 to -100 bp from translation starting site played an important role for TGF-α response. Site-directed mutagenesis and gel mobility shift assay indicated that two Sp1 binding sequences residing at -158 to -150 bp and -123 to -114 bp were responsible for the TGF-α in activation of human 12-lipoxygenase gene transcription. Expression of Sp1, but not Sp3, stimulated the promoter activity of 12-lipoxygenase in SL2 cells, indicating that the binding of Sp1 with Sp1 binding sequences played a significant role in the regulation of 12-lipoxygenase gene. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)105-110
Number of pages6
JournalFEBS Letters
Volume455
Issue number1-2
DOIs
Publication statusPublished - 1999 Jul 16

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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