Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance

Alan Yi Hui Hsu; Shang Rung Wu; Jih Jin Tsai; Po Lin Chen; Ya Ping Chen; Tsai Yun Chen; Yu Chih Lo; Tzu Chuan Ho; Meed Lee; Min Ting Chen; Yen Chi Chiu; Guey Chuen Perng

doi:10.1038/srep17990

Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance

Alan Yi Hui Hsu, Shang Rung Wu, Jih Jin Tsai, Po Lin Chen, Ya Ping Chen, Tsai Yun Chen, Yu Chih Lo, Tzu Chuan Ho, Meed Lee, Min Ting Chen, Yen Chi Chiu, Guey Chuen Perng

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13 Citations (Scopus)

Abstract

The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.

Original language	English
Article number	17990
Journal	Scientific reports
Volume	5
DOIs	https://doi.org/10.1038/srep17990
Publication status	Published - 2015 Dec 11

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@article{11883096073c4b0d812f7f27fe89ce5e,

title = "Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance",

abstract = "The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a {"}sunny-side up egg{"} appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.",

author = "Hsu, {Alan Yi Hui} and Wu, {Shang Rung} and Tsai, {Jih Jin} and Chen, {Po Lin} and Chen, {Ya Ping} and Chen, {Tsai Yun} and Lo, {Yu Chih} and Ho, {Tzu Chuan} and Meed Lee and Chen, {Min Ting} and Chiu, {Yen Chi} and Perng, {Guey Chuen}",

note = "Funding Information: This research was, in part, supported by the Ministry of Education, Taiwan, R.O.C. The Aim for the Top University Project to the National Cheng Kung University (NCKU) ,and National Science Council Grant (NSC 102-2320-B-006–049 and MOST 103 - 2320 - B - 006 - 030 - MY3 (GCP), (NSC101-2628-B-006 -002 -MY3) (SRW), and (NSC 99-2745-B-037-002) (JJT) and (NSC 101-2311-B-006-008-MY3) (YCL).",

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doi = "10.1038/srep17990",

language = "English",

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AU - Hsu, Alan Yi Hui

AU - Wu, Shang Rung

AU - Tsai, Jih Jin

AU - Chen, Po Lin

AU - Chen, Ya Ping

AU - Chen, Tsai Yun

AU - Lo, Yu Chih

AU - Ho, Tzu Chuan

AU - Lee, Meed

AU - Chen, Min Ting

AU - Chiu, Yen Chi

AU - Perng, Guey Chuen

N1 - Funding Information: This research was, in part, supported by the Ministry of Education, Taiwan, R.O.C. The Aim for the Top University Project to the National Cheng Kung University (NCKU) ,and National Science Council Grant (NSC 102-2320-B-006–049 and MOST 103 - 2320 - B - 006 - 030 - MY3 (GCP), (NSC101-2628-B-006 -002 -MY3) (SRW), and (NSC 99-2745-B-037-002) (JJT) and (NSC 101-2311-B-006-008-MY3) (YCL).

PY - 2015/12/11

Y1 - 2015/12/11

N2 - The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.

AB - The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.

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Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance

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