Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance

Alan Yi Hui Hsu, Shang-Rung Wu, Jih Jin Tsai, Po-Lin Chen, Ya-Ping Chen, Tsai-Yun Chen, Yu-Chih Lo, Tzu Chuan Ho, Meed Lee, Min Ting Chen, Yen Chi Chiu, Guey-Chuen Perng

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.

Original languageEnglish
Article number17990
JournalScientific reports
Volume5
DOIs
Publication statusPublished - 2015 Dec 11

Fingerprint

Dengue
Dengue Virus
Vero Cells
Virion
Dengue Vaccines
Vaccines
Viral Envelope Proteins
Viral RNA
Neutralizing Antibodies
Ovum
Immunity
Bone Marrow
Cell Membrane
Serum
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{11883096073c4b0d812f7f27fe89ce5e,
title = "Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance",
abstract = "The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a {"}sunny-side up egg{"} appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.",
author = "Hsu, {Alan Yi Hui} and Shang-Rung Wu and Tsai, {Jih Jin} and Po-Lin Chen and Ya-Ping Chen and Tsai-Yun Chen and Yu-Chih Lo and Ho, {Tzu Chuan} and Meed Lee and Chen, {Min Ting} and Chiu, {Yen Chi} and Guey-Chuen Perng",
year = "2015",
month = "12",
day = "11",
doi = "10.1038/srep17990",
language = "English",
volume = "5",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",

}

Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance. / Hsu, Alan Yi Hui; Wu, Shang-Rung; Tsai, Jih Jin; Chen, Po-Lin; Chen, Ya-Ping; Chen, Tsai-Yun; Lo, Yu-Chih; Ho, Tzu Chuan; Lee, Meed; Chen, Min Ting; Chiu, Yen Chi; Perng, Guey-Chuen.

In: Scientific reports, Vol. 5, 17990, 11.12.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Infectious dengue vesicles derived from CD61+ cells in acute patient plasma exhibited a diaphanous appearance

AU - Hsu, Alan Yi Hui

AU - Wu, Shang-Rung

AU - Tsai, Jih Jin

AU - Chen, Po-Lin

AU - Chen, Ya-Ping

AU - Chen, Tsai-Yun

AU - Lo, Yu-Chih

AU - Ho, Tzu Chuan

AU - Lee, Meed

AU - Chen, Min Ting

AU - Chiu, Yen Chi

AU - Perng, Guey-Chuen

PY - 2015/12/11

Y1 - 2015/12/11

N2 - The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.

AB - The levels of neutralizing antibody to a pathogen are an effective indicator to predict efficacy of a vaccine in trial. And yet not all the trial vaccines are in line with the theory. Using dengue virus (DENV) to investigate the viral morphology affecting the predictive value, we evaluated the viral morphology in acute dengue plasma compared to that of Vero cells derived DENV. The virions in plasma were infectious and heterogeneous in shape with a "sunny-side up egg" appearance, viral RNA was enclosed with CD61+ cell-derived membrane interspersed by the viral envelope protein, defined as dengue vesicles. The unique viral features were also observed from ex vivo infected human bone marrow. Dengue vesicles were less efficiently neutralized by convalescent patient serum, compared to virions produced from Vero cells. Our results exhibit a reason why potencies of protective immunity fail in vivo and significantly impact dengue vaccine and drug development.

UR - http://www.scopus.com/inward/record.url?scp=84949667668&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949667668&partnerID=8YFLogxK

U2 - 10.1038/srep17990

DO - 10.1038/srep17990

M3 - Article

VL - 5

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 17990

ER -