Infiltrated cells in exprimental allergic encephalomyelitis by additional intracerebral injection in myelin-basic-protein-sensitized B6 mice

Tzong Shiann Ho, Chia Ying Tsai, Nina Tsao, Nan Haw Chow, Huan Yao Lei

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We previously reported that murine experimental allergic encephalomyelitis can be induced by an additional intraperitoneal and intracerebral (i.c.) restimulation in resistant B6 mice after standard immunization with myelin antigens in complete Freund's adjuvant and Bordetella pertussis coadjuvant. Neutrophils infiltrated into perivascular spaces at 12 h, followed by mononuclear cells 24 h after i.c. injection. In this study, we report that the i.c. injection induced the expression of intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). The kinetic expression of ICAM-1 or VCAM-1 on brain endothelial cells paralleled the infiltration of neutrophils and mononuclear cells, respectively. The infiltrated lymphocytes also expressed very late antigen-4 (VLA-4) molecules. The microvascular endothelial cells were positive for VCAM-1, whereas the surrounding mononuclear cells were VLA-4 positive. Furthermore, we found a unique subpopulation of cells with characteristics of CD4-CD8-V(β)+ markers. The kinetic studies of this population showed that these cells were transiently depleted from 12 to 24 h after i.c. challenge (before the development of clinical symptoms) in cervical lymph nodes. These CD4-CD8-V(β)8+ cells can be expanded by in vitro cultue with myelin basic protein or IL-2. No significant changes of CD4+/CD8+ cells were noted. CD4+CD8-CD3+ cells were also found in brain by double histochemical stains and were the major infiltrating cells at 24 or 48 h after i.c. challenge.

Original languageEnglish
Pages (from-to)300-307
Number of pages8
JournalJournal of biomedical science
Volume4
Issue number6
DOIs
Publication statusPublished - 1997 Dec 1

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology
  • Biochemistry, medical
  • Pharmacology (medical)

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