Inhibition of bovine herpesvirus-4 replication in endothelial cells by arsenite

Shinn Jong Jiang, Tsun Mei Lin, Guey Yueh Shi, Hock Liew Eng, Hsiao Yen Chen, Hua Lin Wu

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1 Citation (Scopus)


The effect of arsenite pretreatment on bovine herpesvirus-4 (BHV-4) replication in bovine arterial endothelial (BAE) cells was studied. BHV-4 infectivity, including IE-2 expression, DNA replication and viral yield, were significantly reduced at nontoxic concentrations of arsenite in which cellular DNA synthesis or cell viability of BAE cells were not affected under resting and confluent conditions. This effect was accompanied by the induction of heat shock protein 70 (HSP70) and an interrupted cell cycle (causing cell cultures to accumulate at the S and G2/M phases). Actinomycin D inhibited the induction of HSP70 and reduced arsenite antiviral activity. In conclusion, cellular stress response induced by arsenite in BAE cells inhibited replication of BHV-4, and probably resulted from the induction of HSP70 and interference of cell cycle progression.

Original languageEnglish
Pages (from-to)167-175
Number of pages9
JournalAntiviral Research
Issue number3
Publication statusPublished - 2004 Sep

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Virology

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