Inhibition of Ca2+-activated and voltage-dependent K+ currents by 2-mercaptophenyl-1,4-naphthoquinone in pituitary GH3 cells: Contribution to its antiproliferative effect

Mei Han Huang, Sheng-Nan Wu, Chi Pien Chen, Ai Yu Shen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Quinones have been shown to possess antineoplastic activity; however, their effects on ionic currents remain unclear. The effects of 2-mercaptophenyl-1,4-naphthoquinone (2-MPNQ), menadione (MD) and 1,4-naphthoquinone (1,4 NQ) on cell proliferation and ionic currents in pituitary GH3 lactotrophs were investigated in this study. 2-MPNQ was more potent than menadione or 1,4-naphthoquinone in inhibiting the growth of GH3 cells. 2-MPNQ decreased cell proliferation in a concentration-dependent manner with an IC50 value of 3 μM. In whole-cell recording experiments, 2-MPNQ reversibly caused an inhibition of Ca2+-activated K+ current (IK(Ca)) in a concentration-dependent manner. The IC50 value for 2-MPNQ-induced inhibition of IK(Ca) was 7 μM. In the inside-out configuration of single channel recording, 2-MPNQ (30 μM) applied intracellularly suppressed the activity of large-conductance Ca2+-activated K+ (BKCa) channels but did not modify single channel conductance. Menadione (30 μM) had no effect on the channel activity, whereas 1,4-naphthoquinone (30 μM) suppressed it by about 26%. Both 2-MPNQ and thimerosal suppressed the dithiothreitol-stimulated channel activity. 2-MPNQ also blocked voltage-dependent K+ currents, but it produced a slight reduction of L-type Ca2+ inward current. However, unlike E-4031, 2-MPNQ (30 μM) did not suppress inwardly rectifying K+ current present in GH3 cells. Under the current clamp configuration, the presence of 2-MPNQ (30 μM) depolarized the cells, and increased the frequency and duration of spontaneous action potentials. The 2-MPNQ-mediated inhibition of K+ currents would affect hormone secretion and cell excitability. The blockade of these ionic channels by 2-MPNQ may partly explain its inhibitory effect on the proliferation of GH3 cells.

Original languageEnglish
Pages (from-to)1185-1203
Number of pages19
JournalLife Sciences
Volume70
Issue number10
DOIs
Publication statusPublished - 2002 Jan 25

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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