Inorganic phosphate uptake in intact vacuoles isolated from suspension-cultured cells of Catharanthus roseus (L.) G. Don under varying Pi status

Miwa Ohnishi, Tetsuro Mimura, Tomoko Tsujimura, Naoto Mitsuhashi, Setsuko Washitani-Nemoto, Masayoshi Maeshima, Enrico Martinoia

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13 Citations (Scopus)

Abstract

Inorganic phosphate (Pi) uptake across the vacuolar membrane of intact vacuoles isolated from Catharanthus roseus suspension-cultured cells was measured. Under low Pi status, Pi uptake into the vacuole was strongly activated compared to high Pi status. Since Pi uptake across the vacuolar membrane is correlated with H+ pumping, we examined the dependency of H + pumping on plant Pi status. Both H+ pumping and the activities of the vacuolar H+-pumps, the V-type H+-ATPase and the H+-PPase were enhanced under low Pi status. Despite this increase in H+ pumping, Western blot analysis showed no distinct increase in the amount of proton pump proteins. Possible mechanisms for the activation of Pi uptake into the vacuole under low Pi status are discussed.

Original languageEnglish
Pages (from-to)711-718
Number of pages8
JournalPlanta
Volume225
Issue number3
DOIs
Publication statusPublished - 2007 Feb

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

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