TY - JOUR
T1 - Insights into gene regulation of the halovirus His2 infecting Haloarcula hispanica
AU - Lee, Sonny T.M.
AU - Ding, Jiun Yan
AU - Chiang, Pei Wen
AU - Dyall-Smith, Mike
AU - Tang, Sen Lin
N1 - Funding Information:
We would like to thank the?Biodiversity Research Center, Academia Sinica for their financial and facilities support.
Publisher Copyright:
© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
PY - 2020/5/1
Y1 - 2020/5/1
N2 - Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression in both host and viral genes. His2 gene expression occurred in three main phases (early, middle, and late), and by 4.5 hr p.i. the majority of genes were actively transcribed, including those encoding the major structural proteins. Eighty host genes were differentially regulated ≥twofold postinfection, with most of them predicted to be involved in transport, translation, and metabolism. Differentially expressed host genes could also be grouped into early-, middle-, and late-expressed genes based on the timing of their up- and downregulation postinfection. The altered host transcriptional pattern suggests regulation by His2 infection, which may reprogram host metabolism to facilitate its own DNA replication and propagation. This study enhances the characterization of many hypothetical viral genes and provides insights into the interaction between His2 and its host.
AB - Gene expression in Haloarcula hispanica cells infected with the gammapleolipovirus His2 was studied using a custom DNA microarray. Total RNA from cells sampled at 0, 1, 2, 3, and 4.5 hr postinfection was reverse-transcribed into labeled cDNA and hybridized to microarrays, revealing temporal and differential expression in both host and viral genes. His2 gene expression occurred in three main phases (early, middle, and late), and by 4.5 hr p.i. the majority of genes were actively transcribed, including those encoding the major structural proteins. Eighty host genes were differentially regulated ≥twofold postinfection, with most of them predicted to be involved in transport, translation, and metabolism. Differentially expressed host genes could also be grouped into early-, middle-, and late-expressed genes based on the timing of their up- and downregulation postinfection. The altered host transcriptional pattern suggests regulation by His2 infection, which may reprogram host metabolism to facilitate its own DNA replication and propagation. This study enhances the characterization of many hypothetical viral genes and provides insights into the interaction between His2 and its host.
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U2 - 10.1002/mbo3.1016
DO - 10.1002/mbo3.1016
M3 - Article
C2 - 32212320
AN - SCOPUS:85082189674
VL - 9
JO - MicrobiologyOpen
JF - MicrobiologyOpen
SN - 2045-8827
IS - 5
M1 - e1016
ER -