TY - JOUR
T1 - Instrument-Free Detection of FXYD3 Using Vial-Based Immunosensor for Earlier and Faster Urothelial Carcinoma Diagnosis
AU - Hsu, Ying Pei
AU - Yang, Hung Wei
AU - Li, Nan Si
AU - Chen, Ying Tzu
AU - Pang, Hao Han
AU - Pang, See Tong
N1 - Funding Information:
The authors acknowledge the funding support from the Ministry of Science and Technology and Chang Gung Memorial Hospital Taiwan, R.O.C. (MOST106-2628-E-110-001-MY3, MOST106-2628-B-110-001-MY4 CMRPG3H1101-2, NMRPG3H6071-2, and CMRPG3G1271-3). We would also like to thank the Chang Gung Memorial Hospital Microscopy Core Laboratory for their assistance with SEM.
Funding Information:
The authors acknowledge the funding support from the Ministry of Science and Technology and Chang Gung Memorial Hospital, Taiwan, R.O.C. (MOST106-2628-E-110-001-MY3, MOST106-2628-B-110-001-MY4, CMRPG3H1101-2, NMRPG3H6071-2, and CMRPG3G1271-3). We would also like to thank the Chang Gung Memorial Hospital Microscopy Core Laboratory for their assistance with SEM.
Publisher Copyright:
Copyright © 2020 American Chemical Society.
PY - 2020/4/24
Y1 - 2020/4/24
N2 - The incidence and 5 year recurrence rate of urothelial carcinomas (UCs), including UC of the bladder (UCB) and upper urinary tract UC (UTUC), have increased annually. There is a great need for a simple and fast point-of-care (POC) test for early diagnosis and amelioration in the survival rate. We present a POC test comprising a new vial-immunosensor, nanoenzyme, and iPhone 7 plus, which detects and quantifies the new biomarker FXYD domain-containing ion transport regulator 3 (FXYD3) in human urine for specific UC screening, tumor-grade classification, and postoperative monitoring by the grayscale value of the photograph taken. The performance of the proposed POC test was then verified using urine from 4 healthy people, 40 UCB patients (10 patients were low-grade and 30 patients were high-grade), and 13 UTUC patients (2 patients were low-grade and 11 patients were high-grade), confirming the accuracy and specificity by comparing the results with those obtained by enzyme-linked immunosorbent assay (ELISA). Moreover, we also designed a correction method that can make the grayscale values calculated by different smartphones close to the values calculated by iPhone 7 plus, resulting in the POC test enabling simple, fast, universal, and portable testing, data storage, and sharing for personal UCs screening and postoperative monitoring.
AB - The incidence and 5 year recurrence rate of urothelial carcinomas (UCs), including UC of the bladder (UCB) and upper urinary tract UC (UTUC), have increased annually. There is a great need for a simple and fast point-of-care (POC) test for early diagnosis and amelioration in the survival rate. We present a POC test comprising a new vial-immunosensor, nanoenzyme, and iPhone 7 plus, which detects and quantifies the new biomarker FXYD domain-containing ion transport regulator 3 (FXYD3) in human urine for specific UC screening, tumor-grade classification, and postoperative monitoring by the grayscale value of the photograph taken. The performance of the proposed POC test was then verified using urine from 4 healthy people, 40 UCB patients (10 patients were low-grade and 30 patients were high-grade), and 13 UTUC patients (2 patients were low-grade and 11 patients were high-grade), confirming the accuracy and specificity by comparing the results with those obtained by enzyme-linked immunosorbent assay (ELISA). Moreover, we also designed a correction method that can make the grayscale values calculated by different smartphones close to the values calculated by iPhone 7 plus, resulting in the POC test enabling simple, fast, universal, and portable testing, data storage, and sharing for personal UCs screening and postoperative monitoring.
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UR - http://www.scopus.com/inward/citedby.url?scp=85084024675&partnerID=8YFLogxK
U2 - 10.1021/acssensors.9b02013
DO - 10.1021/acssensors.9b02013
M3 - Article
C2 - 32162907
AN - SCOPUS:85084024675
SN - 2379-3694
VL - 5
SP - 928
EP - 935
JO - ACS Sensors
JF - ACS Sensors
IS - 4
ER -
