TY - GEN
T1 - Integrated microfluidic chip for fast diagnosis of piscine nodavirus
AU - Lee, S. H.
AU - Ou, M. C.
AU - Chen, T. Y.
AU - Lee, G. B.
PY - 2007
Y1 - 2007
N2 - This paper reports a fast and sensitive method for early diagnosis of piscine nodavirus by using an automated microfluidic system. This system integrate a micro reverse transcription-polymerase chain reaction (RT-PCR) module, a capillary electrophoresis (CE) module, and buried optical fibers for on-line detection. The microfluidic chip is mainly fabricated from bio-compatible materials such as PDMS (polydimethylsiloxane), polymethylmethacrylate (PMMA), and soda-lime glass. With less human intervention, this integrated microfluidic device successfully detects the piscine nodavirus from clinical fish samples. Meanwhile, it shortens the detection time and also reduces the sample as well as reagent consumption. Moreover, the detection limit can be as low as 12.5 copies/μl by using the CE module. The experimental data confirm this integrated system could be a crucial tool for the diagnosis of RNA (ribonucleic acid)-based virus.
AB - This paper reports a fast and sensitive method for early diagnosis of piscine nodavirus by using an automated microfluidic system. This system integrate a micro reverse transcription-polymerase chain reaction (RT-PCR) module, a capillary electrophoresis (CE) module, and buried optical fibers for on-line detection. The microfluidic chip is mainly fabricated from bio-compatible materials such as PDMS (polydimethylsiloxane), polymethylmethacrylate (PMMA), and soda-lime glass. With less human intervention, this integrated microfluidic device successfully detects the piscine nodavirus from clinical fish samples. Meanwhile, it shortens the detection time and also reduces the sample as well as reagent consumption. Moreover, the detection limit can be as low as 12.5 copies/μl by using the CE module. The experimental data confirm this integrated system could be a crucial tool for the diagnosis of RNA (ribonucleic acid)-based virus.
UR - http://www.scopus.com/inward/record.url?scp=50049124171&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50049124171&partnerID=8YFLogxK
U2 - 10.1109/SENSOR.2007.4300287
DO - 10.1109/SENSOR.2007.4300287
M3 - Conference contribution
AN - SCOPUS:50049124171
SN - 1424408423
SN - 9781424408429
T3 - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
SP - 943
EP - 946
BT - TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
T2 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
Y2 - 10 June 2007 through 14 June 2007
ER -