Integrated microfluidic chip for fast diagnosis of piscine nodavirus

S. H. Lee, M. C. Ou, Tzong-Yueh Chen, G. B. Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This paper reports a fast and sensitive method for early diagnosis of piscine nodavirus by using an automated microfluidic system. This system integrate a micro reverse transcription-polymerase chain reaction (RT-PCR) module, a capillary electrophoresis (CE) module, and buried optical fibers for on-line detection. The microfluidic chip is mainly fabricated from bio-compatible materials such as PDMS (polydimethylsiloxane), polymethylmethacrylate (PMMA), and soda-lime glass. With less human intervention, this integrated microfluidic device successfully detects the piscine nodavirus from clinical fish samples. Meanwhile, it shortens the detection time and also reduces the sample as well as reagent consumption. Moreover, the detection limit can be as low as 12.5 copies/μl by using the CE module. The experimental data confirm this integrated system could be a crucial tool for the diagnosis of RNA (ribonucleic acid)-based virus.

Original languageEnglish
Title of host publicationTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems
Pages943-946
Number of pages4
DOIs
Publication statusPublished - 2007 Dec 1
Event4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07 - Lyon, France
Duration: 2007 Jun 102007 Jun 14

Publication series

NameTRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems

Other

Other4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07
CountryFrance
CityLyon
Period07-06-1007-06-14

Fingerprint

Microfluidics
Capillary electrophoresis
Polymerase chain reaction
Polydimethylsiloxane
Transcription
RNA
Viruses
Biomaterials
Lime
Fish
Optical fibers
Glass

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering
  • Electrical and Electronic Engineering

Cite this

Lee, S. H., Ou, M. C., Chen, T-Y., & Lee, G. B. (2007). Integrated microfluidic chip for fast diagnosis of piscine nodavirus. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems (pp. 943-946). [4300287] (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300287
Lee, S. H. ; Ou, M. C. ; Chen, Tzong-Yueh ; Lee, G. B. / Integrated microfluidic chip for fast diagnosis of piscine nodavirus. TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. pp. 943-946 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).
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Lee, SH, Ou, MC, Chen, T-Y & Lee, GB 2007, Integrated microfluidic chip for fast diagnosis of piscine nodavirus. in TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems., 4300287, TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems, pp. 943-946, 4th International Conference on Solid-State Sensors, Actuators and Microsystems, TRANSDUCERS and EUROSENSORS '07, Lyon, France, 07-06-10. https://doi.org/10.1109/SENSOR.2007.4300287

Integrated microfluidic chip for fast diagnosis of piscine nodavirus. / Lee, S. H.; Ou, M. C.; Chen, Tzong-Yueh; Lee, G. B.

TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 943-946 4300287 (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - This paper reports a fast and sensitive method for early diagnosis of piscine nodavirus by using an automated microfluidic system. This system integrate a micro reverse transcription-polymerase chain reaction (RT-PCR) module, a capillary electrophoresis (CE) module, and buried optical fibers for on-line detection. The microfluidic chip is mainly fabricated from bio-compatible materials such as PDMS (polydimethylsiloxane), polymethylmethacrylate (PMMA), and soda-lime glass. With less human intervention, this integrated microfluidic device successfully detects the piscine nodavirus from clinical fish samples. Meanwhile, it shortens the detection time and also reduces the sample as well as reagent consumption. Moreover, the detection limit can be as low as 12.5 copies/μl by using the CE module. The experimental data confirm this integrated system could be a crucial tool for the diagnosis of RNA (ribonucleic acid)-based virus.

AB - This paper reports a fast and sensitive method for early diagnosis of piscine nodavirus by using an automated microfluidic system. This system integrate a micro reverse transcription-polymerase chain reaction (RT-PCR) module, a capillary electrophoresis (CE) module, and buried optical fibers for on-line detection. The microfluidic chip is mainly fabricated from bio-compatible materials such as PDMS (polydimethylsiloxane), polymethylmethacrylate (PMMA), and soda-lime glass. With less human intervention, this integrated microfluidic device successfully detects the piscine nodavirus from clinical fish samples. Meanwhile, it shortens the detection time and also reduces the sample as well as reagent consumption. Moreover, the detection limit can be as low as 12.5 copies/μl by using the CE module. The experimental data confirm this integrated system could be a crucial tool for the diagnosis of RNA (ribonucleic acid)-based virus.

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Lee SH, Ou MC, Chen T-Y, Lee GB. Integrated microfluidic chip for fast diagnosis of piscine nodavirus. In TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems. 2007. p. 943-946. 4300287. (TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems). https://doi.org/10.1109/SENSOR.2007.4300287