Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens

Wen Hsin Chang, Sung Yi Yang, Chih Hung Wang, Ming An Tsai, Pei Chyi Wang, Shih Chu Chen, Tzong Yueh Chen, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Detection of fish pathogens is crucial for premium aquaculture species with a high economic value. The current study therefore presents a new microfluidic system for detection of aquaculture pathogens. Pathogen DNA was first purified by using specific nucleotide probes, followed by amplifying pathogen-specific DNA fragments by adopting the loop-mediated-isothermal-amplification (LAMP) process. Furthermore, it can carry out four LAMP reactions for multiple detection of pathogens. Successful demonstration for detection of aquaculture fish pathogens has been achieved by using the proposed microfluidic system.

Original languageEnglish
Title of host publication2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
Pages210-213
Number of pages4
DOIs
Publication statusPublished - 2011 Sep 1
Event2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 - Beijing, China
Duration: 2011 Jun 52011 Jun 9

Publication series

Name2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11

Other

Other2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11
CountryChina
CityBeijing
Period11-06-0511-06-09

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All Science Journal Classification (ASJC) codes

  • Hardware and Architecture
  • Electrical and Electronic Engineering

Cite this

Chang, W. H., Yang, S. Y., Wang, C. H., Tsai, M. A., Wang, P. C., Chen, S. C., ... Lee, G. B. (2011). Integrated microfluidic loop-mediated-isothermal-amplification systems for rapid isolation and detection of aquaculture pathogens. In 2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11 (pp. 210-213). [5969305] (2011 16th International Solid-State Sensors, Actuators and Microsystems Conference, TRANSDUCERS'11). https://doi.org/10.1109/TRANSDUCERS.2011.5969305