Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus

Chih Hung Wang, Kang Yi Lien, Ting Yu Wang, Tzong Yueh Chen, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages812-814
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10-10-0310-10-07

Fingerprint

Aquaculture
Viruses
Microfluidics
Amplification
Tissue
Polymerase chain reaction
Nucleic acids
Transcription
RNA
Assays
Cooling
Control systems
Heating
Temperature

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Wang, C. H., Lien, K. Y., Wang, T. Y., Chen, T. Y., & Lee, G. B. (2010). Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 812-814). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).
Wang, Chih Hung ; Lien, Kang Yi ; Wang, Ting Yu ; Chen, Tzong Yueh ; Lee, Gwo Bin. / Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. pp. 812-814 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).
@inproceedings{91565b1604ee4d39984500c3108f56f2,
title = "Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus",
abstract = "The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.",
author = "Wang, {Chih Hung} and Lien, {Kang Yi} and Wang, {Ting Yu} and Chen, {Tzong Yueh} and Lee, {Gwo Bin}",
year = "2010",
month = "12",
day = "1",
language = "English",
isbn = "9781618390622",
series = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",
pages = "812--814",
booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

}

Wang, CH, Lien, KY, Wang, TY, Chen, TY & Lee, GB 2010, Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, vol. 2, pp. 812-814, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 10-10-03.

Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. / Wang, Chih Hung; Lien, Kang Yi; Wang, Ting Yu; Chen, Tzong Yueh; Lee, Gwo Bin.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 812-814 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

TY - GEN

T1 - Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus

AU - Wang, Chih Hung

AU - Lien, Kang Yi

AU - Wang, Ting Yu

AU - Chen, Tzong Yueh

AU - Lee, Gwo Bin

PY - 2010/12/1

Y1 - 2010/12/1

N2 - The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.

AB - The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.

UR - http://www.scopus.com/inward/record.url?scp=84884391573&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884391573&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84884391573

SN - 9781618390622

T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

SP - 812

EP - 814

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

ER -

Wang CH, Lien KY, Wang TY, Chen TY, Lee GB. Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. 2010. p. 812-814. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).