Integrated microfluidic platform for utilizing aptamer-based ELISA-like assay for simultaneous detection of multiple cardiovascular clinical samples

Anirban Sinha, Priya Gopinathan, Yi Da Chung, Shu Chu Shieh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cardiovascular diseases (CVDs) have been documented as one of the leading causes of deaths with an estimated death toll of 17 million. Early detection may reduce the high risk of sudden deaths associated with it. Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. In this work, we developed an integrated microfluidic platform for the simultaneous detection up to six clinical samples using a highly specific aptamer for recognizing the protein followed by an antibody-based chemiluminescence assay for the detection of CVDs biomarker, N-terminal pro-b-type natriuretic peptide (NT-proBNP) in less than 30 minutes. A fully automated on-chip detection of NT-proBNP was performed using only 15 µL of clinical samples from each patient. It may serve a promising tool for CVDs monitoring and diagnosis.

Original languageEnglish
Title of host publication22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
PublisherChemical and Biological Microsystems Society
Pages1181-1184
Number of pages4
ISBN (Electronic)9781510897571
Publication statusPublished - 2018
Event22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 - Kaohsiung, Taiwan
Duration: 2018 Nov 112018 Nov 15

Publication series

Name22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Volume2

Conference

Conference22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Country/TerritoryTaiwan
CityKaohsiung
Period18-11-1118-11-15

All Science Journal Classification (ASJC) codes

  • General Chemistry
  • Bioengineering
  • Chemical Engineering (miscellaneous)
  • Control and Systems Engineering

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