Integrated microfluidic system for HIV detection

Jung Hao Wang; Chih Hung Wang; Wei Shuo Ling; Lie Jheng; Shainn-Wei Wang; Gwo Bin Lee

doi:10.1109/MEMSYS.2012.6170346

Integrated microfluidic system for HIV detection

Jung Hao Wang, Chih Hung Wang, Wei Shuo Ling, Lie Jheng, Shainn-Wei Wang, Gwo Bin Lee

Institute of Molecular Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.

Original language	English
Title of host publication	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages	961-964
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170346
Publication status	Published - 2012 May 7
Event	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France Duration: 2012 Jan 29 → 2012 Feb 2

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country	France
City	Paris
Period	12-01-29 → 12-02-02

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/MEMSYS.2012.6170346

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Cite this

Wang, J. H., Wang, C. H., Ling, W. S., Jheng, L., Wang, S-W., & Lee, G. B. (2012). Integrated microfluidic system for HIV detection. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 961-964). [6170346] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170346

@inproceedings{e16a44bd671e4a868300847bf1c58b9e,

title = "Integrated microfluidic system for HIV detection",

abstract = "This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.",

author = "Wang, {Jung Hao} and Wang, {Chih Hung} and Ling, {Wei Shuo} and Lie Jheng and Shainn-Wei Wang and Lee, {Gwo Bin}",

year = "2012",

month = may,

day = "7",

doi = "10.1109/MEMSYS.2012.6170346",

language = "English",

isbn = "9781467303248",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "961--964",

booktitle = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012",

note = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference date: 29-01-2012 Through 02-02-2012",

}

Wang, JH, Wang, CH, Ling, WS, Jheng, L, Wang, S-W & Lee, GB 2012, Integrated microfluidic system for HIV detection. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170346, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 961-964, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 12-01-29. https://doi.org/10.1109/MEMSYS.2012.6170346

Integrated microfluidic system for HIV detection. / Wang, Jung Hao; Wang, Chih Hung; Ling, Wei Shuo; Jheng, Lie; Wang, Shainn-Wei; Lee, Gwo Bin.

2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 961-964 6170346 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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AB - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.

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