TY - GEN
T1 - Integrated microfluidic system for HIV detection
AU - Wang, Jung Hao
AU - Wang, Chih Hung
AU - Ling, Wei Shuo
AU - Jheng, Lie
AU - Wang, Shainn Wei
AU - Lee, Gwo Bin
PY - 2012
Y1 - 2012
N2 - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.
AB - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.
UR - http://www.scopus.com/inward/record.url?scp=84860468523&partnerID=8YFLogxK
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U2 - 10.1109/MEMSYS.2012.6170346
DO - 10.1109/MEMSYS.2012.6170346
M3 - Conference contribution
AN - SCOPUS:84860468523
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 961
EP - 964
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Y2 - 29 January 2012 through 2 February 2012
ER -