Integrated microfluidic system for HIV detection

Jung Hao Wang; Chih Hung Wang; Wei Shuo Ling; Lie Jheng; Shainn Wei Wang; Gwo Bin Lee

doi:10.1109/MEMSYS.2012.6170346

Integrated microfluidic system for HIV detection

Jung Hao Wang, Chih Hung Wang, Wei Shuo Ling, Lie Jheng, Shainn Wei Wang, Gwo Bin Lee

Institute of Molecular Medicine

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

3 Citations (Scopus)

Abstract

This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.

Original language	English
Title of host publication	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages	961-964
Number of pages	4
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170346
Publication status	Published - 2012
Event	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France Duration: 2012 Jan 29 → 2012 Feb 2

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Country/Territory	France
City	Paris
Period	12-01-29 → 12-02-02

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/MEMSYS.2012.6170346

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Wang, J. H., Wang, C. H., Ling, W. S., Jheng, L., Wang, S. W., & Lee, G. B. (2012). Integrated microfluidic system for HIV detection. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 961-964). Article 6170346 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170346

@inproceedings{e16a44bd671e4a868300847bf1c58b9e,

title = "Integrated microfluidic system for HIV detection",

abstract = "This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.",

author = "Wang, {Jung Hao} and Wang, {Chih Hung} and Ling, {Wei Shuo} and Lie Jheng and Wang, {Shainn Wei} and Lee, {Gwo Bin}",

year = "2012",

doi = "10.1109/MEMSYS.2012.6170346",

language = "English",

isbn = "9781467303248",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "961--964",

booktitle = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012",

note = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 ; Conference date: 29-01-2012 Through 02-02-2012",

}

Wang, JH, Wang, CH, Ling, WS, Jheng, L, Wang, SW & Lee, GB 2012, Integrated microfluidic system for HIV detection. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170346, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 961-964, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 12-01-29. https://doi.org/10.1109/MEMSYS.2012.6170346

Integrated microfluidic system for HIV detection. / Wang, Jung Hao; Wang, Chih Hung; Ling, Wei Shuo et al.
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 961-964 6170346 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Integrated microfluidic system for HIV detection

AU - Wang, Jung Hao

AU - Wang, Chih Hung

AU - Ling, Wei Shuo

AU - Jheng, Lie

AU - Wang, Shainn Wei

AU - Lee, Gwo Bin

PY - 2012

Y1 - 2012

N2 - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.

AB - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.

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U2 - 10.1109/MEMSYS.2012.6170346

DO - 10.1109/MEMSYS.2012.6170346

M3 - Conference contribution

AN - SCOPUS:84860468523

SN - 9781467303248

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 961

EP - 964

BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012

T2 - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012

Y2 - 29 January 2012 through 2 February 2012

ER -

Integrated microfluidic system for HIV detection

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