Abstract
This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.
| Original language | English |
|---|---|
| Title of host publication | 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 |
| Pages | 961-964 |
| Number of pages | 4 |
| DOIs | |
| Publication status | Published - 2012 May 7 |
| Event | 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France Duration: 2012 Jan 29 → 2012 Feb 2 |
Publication series
| Name | Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS) |
|---|---|
| ISSN (Print) | 1084-6999 |
Other
| Other | 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 |
|---|---|
| Country | France |
| City | Paris |
| Period | 12-01-29 → 12-02-02 |
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All Science Journal Classification (ASJC) codes
- Electronic, Optical and Magnetic Materials
- Condensed Matter Physics
- Mechanical Engineering
- Electrical and Electronic Engineering
Cite this
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Integrated microfluidic system for HIV detection. / Wang, Jung Hao; Wang, Chih Hung; Ling, Wei Shuo; Jheng, Lie; Wang, Shainn-Wei; Lee, Gwo Bin.
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 961-964 6170346 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Integrated microfluidic system for HIV detection
AU - Wang, Jung Hao
AU - Wang, Chih Hung
AU - Ling, Wei Shuo
AU - Jheng, Lie
AU - Wang, Shainn-Wei
AU - Lee, Gwo Bin
PY - 2012/5/7
Y1 - 2012/5/7
N2 - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.
AB - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.
UR - http://www.scopus.com/inward/record.url?scp=84860468523&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860468523&partnerID=8YFLogxK
U2 - 10.1109/MEMSYS.2012.6170346
DO - 10.1109/MEMSYS.2012.6170346
M3 - Conference contribution
AN - SCOPUS:84860468523
SN - 9781467303248
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 961
EP - 964
BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
ER -