Integrated microfluidic system for HIV detection

Jung Hao Wang, Chih Hung Wang, Wei Shuo Ling, Lie Jheng, Shainn-Wei Wang, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.

Original languageEnglish
Title of host publication2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages961-964
Number of pages4
DOIs
Publication statusPublished - 2012 May 7
Event2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 - Paris, France
Duration: 2012 Jan 292012 Feb 2

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
CountryFrance
CityParis
Period12-01-2912-02-02

Fingerprint

human immunodeficiency virus
Viruses
Microfluidics
infectious diseases
modules
nucleotides
Nucleotides
cultured cells
purification
beads
genes
Purification
Amplification
DNA
deoxyribonucleic acid
Genes
chips
Cells
fragments
probes

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Wang, J. H., Wang, C. H., Ling, W. S., Jheng, L., Wang, S-W., & Lee, G. B. (2012). Integrated microfluidic system for HIV detection. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 961-964). [6170346] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170346
Wang, Jung Hao ; Wang, Chih Hung ; Ling, Wei Shuo ; Jheng, Lie ; Wang, Shainn-Wei ; Lee, Gwo Bin. / Integrated microfluidic system for HIV detection. 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. pp. 961-964 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).
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abstract = "This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.",
author = "Wang, {Jung Hao} and Wang, {Chih Hung} and Ling, {Wei Shuo} and Lie Jheng and Shainn-Wei Wang and Lee, {Gwo Bin}",
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Wang, JH, Wang, CH, Ling, WS, Jheng, L, Wang, S-W & Lee, GB 2012, Integrated microfluidic system for HIV detection. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170346, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 961-964, 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012, Paris, France, 12-01-29. https://doi.org/10.1109/MEMSYS.2012.6170346

Integrated microfluidic system for HIV detection. / Wang, Jung Hao; Wang, Chih Hung; Ling, Wei Shuo; Jheng, Lie; Wang, Shainn-Wei; Lee, Gwo Bin.

2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 961-964 6170346 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods.

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Wang JH, Wang CH, Ling WS, Jheng L, Wang S-W, Lee GB. Integrated microfluidic system for HIV detection. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 961-964. 6170346. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170346