TY - JOUR
T1 - Integrated microfluidic system for rapid detection of influenza H1N1 virus using a sandwich-based aptamer assay
AU - Tseng, Yi Ting
AU - Wang, Chih Hung
AU - Chang, Chih Peng
AU - Lee, Gwo Bin
N1 - Funding Information:
The authors gratefully acknowledge financial support from Ministry of Science and Technology, Taiwan ( MOST 104-2119−E-007-009 ). Partial financial support from the “ Towards A World-class University Project ( 104N2751E1 )” is also greatly appreciated.
Publisher Copyright:
© 2016 Elsevier B.V.
PY - 2016/8/15
Y1 - 2016/8/15
N2 - The rapid spread of influenza-associated H1N1 viruses has caused serious concern in recent years. Therefore, there is an urgent need for the development of automatic, point-of-care devices for rapid diagnosis of the influenza virus. Conventional approaches suffer from several critical issues; notably, they are time-consuming, labor-intensive, and are characterized by relatively low sensitivity. In this work, we present a new approach for fluorescence-based detection of the influenza A H1N1 virus using a sandwich-based aptamer assay that is automatically performed on an integrated microfluidic system. The entire detection process was shortened to 30 min using this chip-based system which is much faster than the conventional viral culture method. The limit of detection was significantly improved to 0.032 hemagglutination unit due to the high affinity and high specificity of the H1N1-specific aptamers. The results showed that the two-aptamer microfluidic system had about 103 times higher sensitivity than the conventional serological diagnosis. It was demonstrated that the developed microfluidic system may play as a powerful tool in the detection of the H1N1 virus.
AB - The rapid spread of influenza-associated H1N1 viruses has caused serious concern in recent years. Therefore, there is an urgent need for the development of automatic, point-of-care devices for rapid diagnosis of the influenza virus. Conventional approaches suffer from several critical issues; notably, they are time-consuming, labor-intensive, and are characterized by relatively low sensitivity. In this work, we present a new approach for fluorescence-based detection of the influenza A H1N1 virus using a sandwich-based aptamer assay that is automatically performed on an integrated microfluidic system. The entire detection process was shortened to 30 min using this chip-based system which is much faster than the conventional viral culture method. The limit of detection was significantly improved to 0.032 hemagglutination unit due to the high affinity and high specificity of the H1N1-specific aptamers. The results showed that the two-aptamer microfluidic system had about 103 times higher sensitivity than the conventional serological diagnosis. It was demonstrated that the developed microfluidic system may play as a powerful tool in the detection of the H1N1 virus.
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U2 - 10.1016/j.bios.2016.03.073
DO - 10.1016/j.bios.2016.03.073
M3 - Article
C2 - 27054814
AN - SCOPUS:84963668042
SN - 0956-5663
VL - 82
SP - 105
EP - 111
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -