Integrated microfluidic system for rapid detection of influenza H1N1 virus using a sandwich-based aptamer assay

Yi Ting Tseng, Chih Hung Wang, Chih-Peng Chang, Gwo Bin Lee

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The rapid spread of influenza-associated H1N1 viruses has caused serious concern in recent years. Therefore, there is an urgent need for the development of automatic, point-of-care devices for rapid diagnosis of the influenza virus. Conventional approaches suffer from several critical issues; notably, they are time-consuming, labor-intensive, and are characterized by relatively low sensitivity. In this work, we present a new approach for fluorescence-based detection of the influenza A H1N1 virus using a sandwich-based aptamer assay that is automatically performed on an integrated microfluidic system. The entire detection process was shortened to 30 min using this chip-based system which is much faster than the conventional viral culture method. The limit of detection was significantly improved to 0.032 hemagglutination unit due to the high affinity and high specificity of the H1N1-specific aptamers. The results showed that the two-aptamer microfluidic system had about 103 times higher sensitivity than the conventional serological diagnosis. It was demonstrated that the developed microfluidic system may play as a powerful tool in the detection of the H1N1 virus.

Original languageEnglish
Pages (from-to)105-111
Number of pages7
JournalBiosensors and Bioelectronics
Volume82
DOIs
Publication statusPublished - 2016 Aug 15

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H1N1 Subtype Influenza A Virus
Microfluidics
Orthomyxoviridae
Viruses
Assays
Point-of-Care Systems
Satellite Viruses
Influenza A virus
Hemagglutination
Human Influenza
Limit of Detection
Fluorescence
Equipment and Supplies
Personnel

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Cite this

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abstract = "The rapid spread of influenza-associated H1N1 viruses has caused serious concern in recent years. Therefore, there is an urgent need for the development of automatic, point-of-care devices for rapid diagnosis of the influenza virus. Conventional approaches suffer from several critical issues; notably, they are time-consuming, labor-intensive, and are characterized by relatively low sensitivity. In this work, we present a new approach for fluorescence-based detection of the influenza A H1N1 virus using a sandwich-based aptamer assay that is automatically performed on an integrated microfluidic system. The entire detection process was shortened to 30 min using this chip-based system which is much faster than the conventional viral culture method. The limit of detection was significantly improved to 0.032 hemagglutination unit due to the high affinity and high specificity of the H1N1-specific aptamers. The results showed that the two-aptamer microfluidic system had about 103 times higher sensitivity than the conventional serological diagnosis. It was demonstrated that the developed microfluidic system may play as a powerful tool in the detection of the H1N1 virus.",
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Integrated microfluidic system for rapid detection of influenza H1N1 virus using a sandwich-based aptamer assay. / Tseng, Yi Ting; Wang, Chih Hung; Chang, Chih-Peng; Lee, Gwo Bin.

In: Biosensors and Bioelectronics, Vol. 82, 15.08.2016, p. 105-111.

Research output: Contribution to journalArticle

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