Integrated microfluidic system for rapid selection of CRP aptamers utilizing systematic evolution of ligands by exponentail enrichment

Chao June Huang, Hsin I. Lin, Shu-Chu Shiesh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Systematic evolution of ligands by exponential enrichment (SELEX) has been a crucial procedure which allows extraction of oligomers with desired binding affinity for a target molecule from an initially random pool of oligonucleotides. It can be used to screen DNA- or RNA-based aptamers which are specific to proteins and biomolecules, making it a promising platform for the study of biomarkers. This study reports a new magnetic-bead-based microfluidic system for performing SELEX in an automatic mode. The developed microsystem is promising for fast selection of aptamers, which is an excellent tool for selection of nucleotides with high affinity for a particular target from a random pool under specific conditions.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages150-152
Number of pages3
ISBN (Print)9780979806421
Publication statusPublished - 2009 Jan 1
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 2009 Nov 12009 Nov 5

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
CountryKorea, Republic of
CityJeju
Period09-11-0109-11-05

Fingerprint

Microfluidics
Ligands
Oligonucleotides
Microsystems
Biomolecules
Biomarkers
Nucleotides
RNA
Oligomers
DNA
Proteins
Molecules

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Huang, C. J., Lin, H. I., Shiesh, S-C., & Lee, G. B. (2009). Integrated microfluidic system for rapid selection of CRP aptamers utilizing systematic evolution of ligands by exponentail enrichment. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 150-152). (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.
Huang, Chao June ; Lin, Hsin I. ; Shiesh, Shu-Chu ; Lee, Gwo Bin. / Integrated microfluidic system for rapid selection of CRP aptamers utilizing systematic evolution of ligands by exponentail enrichment. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. pp. 150-152 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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Huang, CJ, Lin, HI, Shiesh, S-C & Lee, GB 2009, Integrated microfluidic system for rapid selection of CRP aptamers utilizing systematic evolution of ligands by exponentail enrichment. in Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 150-152, 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009, Jeju, Korea, Republic of, 09-11-01.

Integrated microfluidic system for rapid selection of CRP aptamers utilizing systematic evolution of ligands by exponentail enrichment. / Huang, Chao June; Lin, Hsin I.; Shiesh, Shu-Chu; Lee, Gwo Bin.

Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2009. p. 150-152 (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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Huang CJ, Lin HI, Shiesh S-C, Lee GB. Integrated microfluidic system for rapid selection of CRP aptamers utilizing systematic evolution of ligands by exponentail enrichment. In Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2009. p. 150-152. (Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences).