Abstract
Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.
| Original language | English |
|---|---|
| Title of host publication | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 67-68 |
| Number of pages | 2 |
| ISBN (Electronic) | 9780979806490 |
| Publication status | Published - 2016 Jan 1 |
| Event | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland Duration: 2016 Oct 9 → 2016 Oct 13 |
Publication series
| Name | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|
Other
| Other | 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 |
|---|---|
| Country | Ireland |
| City | Dublin |
| Period | 16-10-09 → 16-10-13 |
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All Science Journal Classification (ASJC) codes
- Control and Systems Engineering
Cite this
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Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. / Sinha, Anirban; Chung, Yi Da; Gopinathan, Priya; Hung, Lien Yu; Yang, Ching Hsuan; Shiesh, Shu-Chu; Lee, Gwo Bin.
20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 67-68 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
TY - GEN
T1 - Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers
AU - Sinha, Anirban
AU - Chung, Yi Da
AU - Gopinathan, Priya
AU - Hung, Lien Yu
AU - Yang, Ching Hsuan
AU - Shiesh, Shu-Chu
AU - Lee, Gwo Bin
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.
AB - Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.
UR - http://www.scopus.com/inward/record.url?scp=85014221547&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014221547&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014221547
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 67
EP - 68
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
ER -