Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers

Anirban Sinha, Yi Da Chung, Priya Gopinathan, Lien Yu Hung, Ching Hsuan Yang, Shu-Chu Shiesh, Gwo Bin Lee

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.

Original languageEnglish
Title of host publication20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PublisherChemical and Biological Microsystems Society
Pages67-68
Number of pages2
ISBN (Electronic)9780979806490
Publication statusPublished - 2016 Jan 1
Event20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 - Dublin, Ireland
Duration: 2016 Oct 92016 Oct 13

Publication series

Name20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

Other

Other20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
CountryIreland
CityDublin
Period16-10-0916-10-13

Fingerprint

Biomarkers
Microfluidics
Antibodies
Screening
Ligands
Antigens
Demonstrations
Proteins
Natriuretic Peptides

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Sinha, A., Chung, Y. D., Gopinathan, P., Hung, L. Y., Yang, C. H., Shiesh, S-C., & Lee, G. B. (2016). Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016 (pp. 67-68). (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016). Chemical and Biological Microsystems Society.
Sinha, Anirban ; Chung, Yi Da ; Gopinathan, Priya ; Hung, Lien Yu ; Yang, Ching Hsuan ; Shiesh, Shu-Chu ; Lee, Gwo Bin. / Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. pp. 67-68 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).
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title = "Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers",
abstract = "Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.",
author = "Anirban Sinha and Chung, {Yi Da} and Priya Gopinathan and Hung, {Lien Yu} and Yang, {Ching Hsuan} and Shu-Chu Shiesh and Lee, {Gwo Bin}",
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Sinha, A, Chung, YD, Gopinathan, P, Hung, LY, Yang, CH, Shiesh, S-C & Lee, GB 2016, Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. in 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Chemical and Biological Microsystems Society, pp. 67-68, 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016, Dublin, Ireland, 16-10-09.

Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. / Sinha, Anirban; Chung, Yi Da; Gopinathan, Priya; Hung, Lien Yu; Yang, Ching Hsuan; Shiesh, Shu-Chu; Lee, Gwo Bin.

20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society, 2016. p. 67-68 (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.

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Sinha A, Chung YD, Gopinathan P, Hung LY, Yang CH, Shiesh S-C et al. Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers. In 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016. Chemical and Biological Microsystems Society. 2016. p. 67-68. (20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016).