TY - GEN
T1 - Integrated microfluidic systems for screening of aptamers specific to cardiovascular biomarkers
AU - Sinha, Anirban
AU - Chung, Yi Da
AU - Gopinathan, Priya
AU - Hung, Lien Yu
AU - Yang, Ching Hsuan
AU - Shiesh, Shu Chu
AU - Lee, Gwo Bin
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2016
Y1 - 2016
N2 - Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.
AB - Current biomedical sensing processes vastly rely on antigen-antibody based detection processes, which have limitations in point-of-care applications. Recently, an artificial antibody, aptamer, has emerging as a promising candidate for diagnosis, which could be screened from a process called systematic evolution of ligands by exponential enrichment (SELEX). In this study, we have developed a new, automated, miniaturized SELEX platform for screening of affinity reagents towards protein biomarkers for cardiovascular diseases (CVDs). As a demonstration, we reported for the first time a complete 'on-chip process to screen aptamers for targeting N-terminal pro b-type natriuretic peptide (NT-pro BNP)' on an integrated microfluidic system.
UR - http://www.scopus.com/inward/record.url?scp=85014221547&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014221547&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:85014221547
T3 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
SP - 67
EP - 68
BT - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
PB - Chemical and Biological Microsystems Society
T2 - 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016
Y2 - 9 October 2016 through 13 October 2016
ER -
