TY - JOUR
T1 - Integrating Porous Silicon Nanoneedles within Medical Devices for Nucleic Acid Nanoinjection
AU - Wang, Cong
AU - Gu, Chenlei
AU - Popp, Courtney
AU - Vashisth, Priya
AU - Mustfa, Salman Ahmad
AU - Martella, Davide Alessandro
AU - Spiteri, Chantelle
AU - McLennan, Samuel
AU - Sun, Ningjia
AU - Riddle, Megan
AU - Eide, Cindy R.
AU - Parsons, Maddy
AU - Tolar, Jakub
AU - McGrath, John A.
AU - Chiappini, Ciro
N1 - Publisher Copyright:
© 2024 The Authors. Published by American Chemical Society.
PY - 2024/6/11
Y1 - 2024/6/11
N2 - Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell-nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.
AB - Porous silicon nanoneedles can interface with cells and tissues with minimal perturbation for high-throughput intracellular delivery and biosensing. Typically, nanoneedle devices are rigid, flat, and opaque, which limits their use for topical applications in the clinic. We have developed a robust, rapid, and precise substrate transfer approach to incorporate nanoneedles within diverse substrates of arbitrary composition, flexibility, curvature, transparency, and biodegradability. With this approach, we integrated nanoneedles on medically relevant elastomers, hydrogels, plastics, medical bandages, catheter tubes, and contact lenses. The integration retains the mechanical properties and transfection efficiency of the nanoneedles. Transparent devices enable the live monitoring of cell-nanoneedle interactions. Flexible devices interface with tissues for efficient, uniform, and sustained topical delivery of nucleic acids ex vivo and in vivo. The versatility of this approach highlights the opportunity to integrate nanoneedles within existing medical devices to develop advanced platforms for topical delivery and biosensing.
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U2 - 10.1021/acsnano.4c00206
DO - 10.1021/acsnano.4c00206
M3 - Article
C2 - 38726598
AN - SCOPUS:85192856203
SN - 1936-0851
VL - 18
SP - 14938
EP - 14953
JO - ACS nano
JF - ACS nano
IS - 23
ER -