Interaction of Mercuric Ions with the Bacterial Growth Medium and Its Effects on Enzymatic Reduction of Mercury

Jo‐Shu ‐S Chang; Juan Hong; Oladele A. Ogunseitan; Betty H. Olson

doi:10.1021/bp00023a012

Interaction of Mercuric Ions with the Bacterial Growth Medium and Its Effects on Enzymatic Reduction of Mercury

Jo‐Shu ‐S Chang, Juan Hong, Oladele A. Ogunseitan, Betty H. Olson

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The interaction of Hg²⁺ with components of Luria‐Bertani broth (LB), used to grow the mercury‐resistant strain Pseudomonas aeruginosa PU21 (Rip64), was studied using cold vapor atomic absorption spectrometry and liquid scintillation counting. The time course analysis of free Hg²⁺ and total Hg in LB solutions showed that 30–40% of Hg²⁺ formed complexes with tryptone and yeast extract within 140 h. Media containing free or complexed Hg²⁺ were treated with cell‐free mercuric reductase as well as with growing cells to investigate the effect of the Hg²⁺‐LB interaction on mercury reduction. In both assays, it was found that the complexed Hg²⁺ could not be reduced by mercuric reductase or P. aeruginosa PU21 (Rip64).

Original language	English
Pages (from-to)	526-532
Number of pages	7
Journal	Biotechnology Progress
Volume	9
Issue number	5
DOIs	https://doi.org/10.1021/bp00023a012
Publication status	Published - 1993

All Science Journal Classification (ASJC) codes

Biotechnology

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10.1021/bp00023a012

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title = "Interaction of Mercuric Ions with the Bacterial Growth Medium and Its Effects on Enzymatic Reduction of Mercury",

abstract = "The interaction of Hg2+ with components of Luria‐Bertani broth (LB), used to grow the mercury‐resistant strain Pseudomonas aeruginosa PU21 (Rip64), was studied using cold vapor atomic absorption spectrometry and liquid scintillation counting. The time course analysis of free Hg2+ and total Hg in LB solutions showed that 30–40% of Hg2+ formed complexes with tryptone and yeast extract within 140 h. Media containing free or complexed Hg2+ were treated with cell‐free mercuric reductase as well as with growing cells to investigate the effect of the Hg2+‐LB interaction on mercury reduction. In both assays, it was found that the complexed Hg2+ could not be reduced by mercuric reductase or P. aeruginosa PU21 (Rip64).",

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AU - Chang, Jo‐Shu ‐S

AU - Hong, Juan

AU - Ogunseitan, Oladele A.

AU - Olson, Betty H.

PY - 1993

Y1 - 1993

N2 - The interaction of Hg2+ with components of Luria‐Bertani broth (LB), used to grow the mercury‐resistant strain Pseudomonas aeruginosa PU21 (Rip64), was studied using cold vapor atomic absorption spectrometry and liquid scintillation counting. The time course analysis of free Hg2+ and total Hg in LB solutions showed that 30–40% of Hg2+ formed complexes with tryptone and yeast extract within 140 h. Media containing free or complexed Hg2+ were treated with cell‐free mercuric reductase as well as with growing cells to investigate the effect of the Hg2+‐LB interaction on mercury reduction. In both assays, it was found that the complexed Hg2+ could not be reduced by mercuric reductase or P. aeruginosa PU21 (Rip64).

AB - The interaction of Hg2+ with components of Luria‐Bertani broth (LB), used to grow the mercury‐resistant strain Pseudomonas aeruginosa PU21 (Rip64), was studied using cold vapor atomic absorption spectrometry and liquid scintillation counting. The time course analysis of free Hg2+ and total Hg in LB solutions showed that 30–40% of Hg2+ formed complexes with tryptone and yeast extract within 140 h. Media containing free or complexed Hg2+ were treated with cell‐free mercuric reductase as well as with growing cells to investigate the effect of the Hg2+‐LB interaction on mercury reduction. In both assays, it was found that the complexed Hg2+ could not be reduced by mercuric reductase or P. aeruginosa PU21 (Rip64).

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Interaction of Mercuric Ions with the Bacterial Growth Medium and Its Effects on Enzymatic Reduction of Mercury

Abstract

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