Interleukin-20 induced cell death in renal epithelial cells and was associated with acute renal failure

H. H. Li, Yu-Hsiang Hsu, C. C. Wei, P. T. Lee, W. C. Chen, Ming-Shi Chang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Acute renal failure is an abrupt decrease in renal function. Interleukin (IL)-10 inhibits ischemic and cisplatin-induced acute renal failure. We aimed to determine whether IL-20 affects renal tubular epithelial cells and is associated with acute renal failure. We analyzed the expression of IL-20 and its receptor (R) in the kidneys of rats with HgCl2-induced acute renal failure. Reverse transcription-PCR showed upregulated IL-20, and its receptors and immunohistochemical staining showed strongly expressed IL-20 protein in proximal tubular epithelial cells. We analyzed human proximal tubular epithelial (HK-2) cells, which expressed both IL-20 and its receptors. IL-20 specifically induced mitochondria-dependent apoptosis by activating caspase 9 in HK-2 cells. IL-20 also activated c-Jun N-terminal kinase and extracellular signal-regulated kinase 1/2, the downstream signals implicated in the apoptosis of HK-2 cells. Furthermore, IL-20 upregulated the transcripts of transforming growth factor (TGF)-β1, a critical mediator of renal injury. In hypoxic HK-2 cells, IL-20 and IL-22R1 transcripts increased, and IL-20 upregulated IL-1β transcripts. In vivo study further demonstrated that anti-IL-20 antibody reduced the expression of TGF-β1 and IL-1β and the number of damaged tubular cells in the kidneys of rats with acute renal failure. We concluded that IL-20 may be involved in the injury of renal epithelial cells in acute renal failure.

Original languageEnglish
Pages (from-to)395-404
Number of pages10
JournalGenes and Immunity
Volume9
Issue number5
DOIs
Publication statusPublished - 2008 Jul 1

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Acute Kidney Injury
Cell Death
Epithelial Cells
Kidney
Transforming Growth Factors
Interleukin-1
Apoptosis
interleukin 20
Mercuric Chloride
Mitogen-Activated Protein Kinase 3
Caspase 9
JNK Mitogen-Activated Protein Kinases
Interleukins
Mitogen-Activated Protein Kinase 1
Wounds and Injuries
Interleukin-10
Cisplatin
Reverse Transcription
Mitochondria
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Immunology
  • Genetics
  • Genetics(clinical)

Cite this

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title = "Interleukin-20 induced cell death in renal epithelial cells and was associated with acute renal failure",
abstract = "Acute renal failure is an abrupt decrease in renal function. Interleukin (IL)-10 inhibits ischemic and cisplatin-induced acute renal failure. We aimed to determine whether IL-20 affects renal tubular epithelial cells and is associated with acute renal failure. We analyzed the expression of IL-20 and its receptor (R) in the kidneys of rats with HgCl2-induced acute renal failure. Reverse transcription-PCR showed upregulated IL-20, and its receptors and immunohistochemical staining showed strongly expressed IL-20 protein in proximal tubular epithelial cells. We analyzed human proximal tubular epithelial (HK-2) cells, which expressed both IL-20 and its receptors. IL-20 specifically induced mitochondria-dependent apoptosis by activating caspase 9 in HK-2 cells. IL-20 also activated c-Jun N-terminal kinase and extracellular signal-regulated kinase 1/2, the downstream signals implicated in the apoptosis of HK-2 cells. Furthermore, IL-20 upregulated the transcripts of transforming growth factor (TGF)-β1, a critical mediator of renal injury. In hypoxic HK-2 cells, IL-20 and IL-22R1 transcripts increased, and IL-20 upregulated IL-1β transcripts. In vivo study further demonstrated that anti-IL-20 antibody reduced the expression of TGF-β1 and IL-1β and the number of damaged tubular cells in the kidneys of rats with acute renal failure. We concluded that IL-20 may be involved in the injury of renal epithelial cells in acute renal failure.",
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Interleukin-20 induced cell death in renal epithelial cells and was associated with acute renal failure. / Li, H. H.; Hsu, Yu-Hsiang; Wei, C. C.; Lee, P. T.; Chen, W. C.; Chang, Ming-Shi.

In: Genes and Immunity, Vol. 9, No. 5, 01.07.2008, p. 395-404.

Research output: Contribution to journalArticle

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