Invasive hypermucoid variant of group A Streptococcus is defective in growth and susceptible to DNA-damaging treatments

Chuan Chiang-Ni, Po Xing Zheng, Shu-Ying Wang, Pei-Jane Tsai, Chih Feng Kuo, Woei-Jer Chuang, Yee-Shin Lin, Ching-Chuan Liu, Jiunn Jong Wu

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Hyaluronic acid capsule is one of the most important virulence factors of group A Streptococcus (GAS). Over-production of capsule has been thought to enhance GAS virulence during infections. However, although the increased of capsule expression associates with increased bacterial virulence and invasive ability, over-production of capsule has not often been observed among clinical isolates. In the present study, we identified two mucoid emm12 type isolates that can convert to the hypermucoid morphology under both in vitro and in vivo conditions. Consistent with previous studies, hypermucoid variants are more invasive in the mouse air-pouch infection model. However, one of the hypermucoid variants showed a growth-defective phenotype in regular broth culture conditions and is significantly more susceptible to various DNA-damaging treatments when compared with the mucoid variant. These properties of the hypermucoid variant may be adverse factors inhibiting its adaptation to the host environment during infections.

Original languageEnglish
Pages (from-to)194-201
Number of pages8
JournalPathogens and Disease
Volume70
Issue number2
DOIs
Publication statusPublished - 2014 Jan 1

Fingerprint

Streptococcus
Capsules
DNA
Growth
Virulence
Infection
Virulence Factors
Hyaluronic Acid
Air
Phenotype

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology and Microbiology(all)
  • Microbiology (medical)
  • Infectious Diseases

Cite this

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abstract = "Hyaluronic acid capsule is one of the most important virulence factors of group A Streptococcus (GAS). Over-production of capsule has been thought to enhance GAS virulence during infections. However, although the increased of capsule expression associates with increased bacterial virulence and invasive ability, over-production of capsule has not often been observed among clinical isolates. In the present study, we identified two mucoid emm12 type isolates that can convert to the hypermucoid morphology under both in vitro and in vivo conditions. Consistent with previous studies, hypermucoid variants are more invasive in the mouse air-pouch infection model. However, one of the hypermucoid variants showed a growth-defective phenotype in regular broth culture conditions and is significantly more susceptible to various DNA-damaging treatments when compared with the mucoid variant. These properties of the hypermucoid variant may be adverse factors inhibiting its adaptation to the host environment during infections.",
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Invasive hypermucoid variant of group A Streptococcus is defective in growth and susceptible to DNA-damaging treatments. / Chiang-Ni, Chuan; Zheng, Po Xing; Wang, Shu-Ying; Tsai, Pei-Jane; Kuo, Chih Feng; Chuang, Woei-Jer; Lin, Yee-Shin; Liu, Ching-Chuan; Wu, Jiunn Jong.

In: Pathogens and Disease, Vol. 70, No. 2, 01.01.2014, p. 194-201.

Research output: Contribution to journalArticle

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