Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer

Hsiang Sheng Wen, Lai Fu Tsai, Chien Wei Liu, Yu Chang Tyan, I. Long Shy, Bau Tong Dai, Pao Chi Liao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.

Original languageEnglish
Title of host publication2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
Pages1015-1017
Number of pages3
DOIs
Publication statusPublished - 2007 Dec 1
Event2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007 - Hong Kong, China
Duration: 2007 Aug 22007 Aug 5

Publication series

Name2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings

Other

Other2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
CountryChina
CityHong Kong
Period07-08-0207-08-05

Fingerprint

Mass spectrometers
Nanostructured materials
Peptides
mass spectrometers
Ionization
peptides
Desorption
desorption
ionization
serums
Carbon
Mass spectrometry
Lasers
carbon
mass spectroscopy
matrices
Iron
lasers
iron
Fullerenes

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

Cite this

Wen, H. S., Tsai, L. F., Liu, C. W., Tyan, Y. C., Shy, I. L., Dai, B. T., & Liao, P. C. (2007). Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. In 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings (pp. 1015-1017). [4601355] (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings). https://doi.org/10.1109/NANO.2007.4601355
Wen, Hsiang Sheng ; Tsai, Lai Fu ; Liu, Chien Wei ; Tyan, Yu Chang ; Shy, I. Long ; Dai, Bau Tong ; Liao, Pao Chi. / Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings. 2007. pp. 1015-1017 (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings).
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abstract = "The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.",
author = "Wen, {Hsiang Sheng} and Tsai, {Lai Fu} and Liu, {Chien Wei} and Tyan, {Yu Chang} and Shy, {I. Long} and Dai, {Bau Tong} and Liao, {Pao Chi}",
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Wen, HS, Tsai, LF, Liu, CW, Tyan, YC, Shy, IL, Dai, BT & Liao, PC 2007, Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. in 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings., 4601355, 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings, pp. 1015-1017, 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Hong Kong, China, 07-08-02. https://doi.org/10.1109/NANO.2007.4601355

Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. / Wen, Hsiang Sheng; Tsai, Lai Fu; Liu, Chien Wei; Tyan, Yu Chang; Shy, I. Long; Dai, Bau Tong; Liao, Pao Chi.

2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings. 2007. p. 1015-1017 4601355 (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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N2 - The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.

AB - The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.

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Wen HS, Tsai LF, Liu CW, Tyan YC, Shy IL, Dai BT et al. Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. In 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings. 2007. p. 1015-1017. 4601355. (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings). https://doi.org/10.1109/NANO.2007.4601355