Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer

Hsiang Sheng Wen; Lai Fu Tsai; Chien Wei Liu; Yu Chang Tyan; I. Long Shy; Bau Tong Dai; Pao Chi Liao

doi:10.1109/NANO.2007.4601355

Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer

Hsiang Sheng Wen, Lai Fu Tsai, Chien Wei Liu, Yu Chang Tyan, I. Long Shy, Bau Tong Dai, Pao Chi Liao

Department of Environmental and Occupational Health

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.

Original language	English
Title of host publication	2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings
Pages	1015-1017
Number of pages	3
DOIs	https://doi.org/10.1109/NANO.2007.4601355
Publication status	Published - 2007
Event	2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007 - Hong Kong, China Duration: 2007 Aug 2 → 2007 Aug 5

Publication series

Name	2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings

Other

Other	2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007
Country/Territory	China
City	Hong Kong
Period	07-08-02 → 07-08-05

All Science Journal Classification (ASJC) codes

Electrical and Electronic Engineering
Atomic and Molecular Physics, and Optics

Access to Document

10.1109/NANO.2007.4601355

Cite this

Wen, H. S., Tsai, L. F., Liu, C. W., Tyan, Y. C., Shy, I. L., Dai, B. T., & Liao, P. C. (2007). Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. In 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings (pp. 1015-1017). Article 4601355 (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings). https://doi.org/10.1109/NANO.2007.4601355

Wen, Hsiang Sheng ; Tsai, Lai Fu ; Liu, Chien Wei et al. / Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings. 2007. pp. 1015-1017 (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings).

@inproceedings{fb278dbc0ec9415b8e526e4da4dd5d46,

title = "Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer",

abstract = "The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.",

author = "Wen, \{Hsiang Sheng\} and Tsai, \{Lai Fu\} and Liu, \{Chien Wei\} and Tyan, \{Yu Chang\} and Shy, \{I. Long\} and Dai, \{Bau Tong\} and Liao, \{Pao Chi\}",

year = "2007",

doi = "10.1109/NANO.2007.4601355",

language = "English",

isbn = "1424406080",

series = "2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings",

pages = "1015--1017",

booktitle = "2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings",

note = "2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007 ; Conference date: 02-08-2007 Through 05-08-2007",

}

Wen, HS, Tsai, LF, Liu, CW, Tyan, YC, Shy, IL, Dai, BT & Liao, PC 2007, Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. in 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings., 4601355, 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings, pp. 1015-1017, 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Hong Kong, China, 07-08-02. https://doi.org/10.1109/NANO.2007.4601355

Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. / Wen, Hsiang Sheng; Tsai, Lai Fu; Liu, Chien Wei et al.
2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings. 2007. p. 1015-1017 4601355 (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer

AU - Wen, Hsiang Sheng

AU - Tsai, Lai Fu

AU - Liu, Chien Wei

AU - Tyan, Yu Chang

AU - Shy, I. Long

AU - Dai, Bau Tong

AU - Liao, Pao Chi

PY - 2007

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N2 - The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.

AB - The study presents the mass spectrometry application of carbon nanomaterials in proteomic research. We utilized two spherical carbon nanomaterials, C60 buckyball and iron-cored carbonball (Fe-C), in peptide sequence identification of a serum protein using matrix-assisted laser desorption ionization-time of flight mass spectrometer (MALDI-TOF-MS). The biological samples used in the experiments were enzymatically trypsinized bovine serum albumins (BSA) peptides instead of synthesized small peptide fragments. Experimental results were compared and noticeable improvements in mass spectrometry spectrum were obtained and quantified. In addition, we also utilized EDS and TEM to determine the chemical composition and morphology of nanostructure of iron-cored carbonball.

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DO - 10.1109/NANO.2007.4601355

M3 - Conference contribution

AN - SCOPUS:52949147181

SN - 1424406080

SN - 9781424406081

T3 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings

SP - 1015

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BT - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings

T2 - 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007

Y2 - 2 August 2007 through 5 August 2007

ER -

Wen HS, Tsai LF, Liu CW, Tyan YC, Shy IL, Dai BT et al. Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer. In 2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings. 2007. p. 1015-1017. 4601355. (2007 7th IEEE International Conference on Nanotechnology - IEEE-NANO 2007, Proceedings). doi: 10.1109/NANO.2007.4601355

Investigation of effects attributed to spherical carbon nanomaterials in proteomic matrix-assisted laser desorption ionization-time of flight mass spectrometer

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