Investigation of flavonoid glycosides from Neolitsea sericea var. aurata via the general method and HPLC-SPE-NMR

Sio Hong Lam, Chien Kuang Chen, Jeng Shu Wang, Shoei Sheng Lee

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

Six flavonoid rhamnosides (1-2, 4-7) were separated from the water-soluble non-alkaloidal portion of the EtOH extract of the leaves of Neolitsea sericea var. aurata using various chromatographic techniques including Sephadex LH-20, centrifugal partition chromatography, and RP-18 Lobar. One additional compound (3) besides these six was identified by application of HPLC-SPE-NMR in a flavonoid rich fraction. The latter approach consumed only 1.5 mg of samples, theoretically equivalent to 0.2 g of dry leaves. This study demonstrates that HPLC-SPE-NMR has great advantage over the general methods on the aspects of manpower, research time, amounts of plant materials, and consumables in separation and characterization of natural products.

Original languageEnglish
Pages (from-to)449-455
Number of pages7
JournalJournal of the Chinese Chemical Society
Volume55
Issue number2
DOIs
Publication statusPublished - 2008

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

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