Investigations of urethral sphincter activity in mice with bladder hyperalgesia before and after drug administration of gabapentin

Jih Chao Yeh, Rebecca Do, Hanul Choi, Ching Ting Lin, Jia-Jin Chen, Xiaolin Zi, Huiyi H. Chang, Gamal Ghoniem

Research output: Contribution to journalArticle

Abstract

Purpose: This study investigated the effect of gabapentin on lower urinary tract dysfunction focusing on urethral activities and cystitis-induced hyperalgesia in a mouse model of painful bladder syndrome/interstitial cystitis (PBS/IC). The electromyography (EMG) of external urethral sphincter (EUS) was difficult to obtain, but contained useful information to examine the drug effect in mice. Methods: Female C57BL/6J mice were intraperitoneally (ip) administration with either saline or 200 mg/kg of cyclophosphamide (CYP) 48 h before experimental evaluation. Cystitis mice were treated with administration of gabapentin (25 or 50 mg/kg, ip). Cystometry and EUS EMG were obtained and analyzed during continuous bladder infusion. The visceral pain-related visceromotor reflex (VMR) was recorded in response to isotonic bladder distension. Results: Cystitis mice showed shorter inter-contraction intervals and increased occurrence of non-voiding contractions during bladder infusion, with increased VMR during isotonic bladder distension, indicating cystitis-induced bladder hyperalgesia. Gabapentin (50 mg/kg) suppressed effects of CYP on cystometry, but not on EUS EMG activity, during bladder infusion. The effect on urodynamic recordings lasted 4 h. VMR was significantly reduced by gabapentin. Conclusions: The present study showed that CYP-induced cystitis in mice is a model of visceral hyperalgesia affecting detrusor contractions, not urethral activations. The technique of using EUS EMG to evaluate the drug effects on urethral activities is novel and useful for future investigations. Gabapentin can be as a potential treatment for detrusor overactivity and PBS/IC.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalInternational Urology and Nephrology
Volume51
Issue number1
DOIs
Publication statusPublished - 2019 Jan 3

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Hyperalgesia
Urethra
Cystitis
Interstitial Cystitis
Urinary Bladder
Electromyography
Pharmaceutical Preparations
Cyclophosphamide
Reflex
Visceral Pain
Urodynamics
Urinary Tract
Inbred C57BL Mouse
gabapentin

All Science Journal Classification (ASJC) codes

  • Nephrology
  • Urology

Cite this

Yeh, Jih Chao ; Do, Rebecca ; Choi, Hanul ; Lin, Ching Ting ; Chen, Jia-Jin ; Zi, Xiaolin ; Chang, Huiyi H. ; Ghoniem, Gamal. / Investigations of urethral sphincter activity in mice with bladder hyperalgesia before and after drug administration of gabapentin. In: International Urology and Nephrology. 2019 ; Vol. 51, No. 1. pp. 53-59.
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Investigations of urethral sphincter activity in mice with bladder hyperalgesia before and after drug administration of gabapentin. / Yeh, Jih Chao; Do, Rebecca; Choi, Hanul; Lin, Ching Ting; Chen, Jia-Jin; Zi, Xiaolin; Chang, Huiyi H.; Ghoniem, Gamal.

In: International Urology and Nephrology, Vol. 51, No. 1, 03.01.2019, p. 53-59.

Research output: Contribution to journalArticle

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T1 - Investigations of urethral sphincter activity in mice with bladder hyperalgesia before and after drug administration of gabapentin

AU - Yeh, Jih Chao

AU - Do, Rebecca

AU - Choi, Hanul

AU - Lin, Ching Ting

AU - Chen, Jia-Jin

AU - Zi, Xiaolin

AU - Chang, Huiyi H.

AU - Ghoniem, Gamal

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N2 - Purpose: This study investigated the effect of gabapentin on lower urinary tract dysfunction focusing on urethral activities and cystitis-induced hyperalgesia in a mouse model of painful bladder syndrome/interstitial cystitis (PBS/IC). The electromyography (EMG) of external urethral sphincter (EUS) was difficult to obtain, but contained useful information to examine the drug effect in mice. Methods: Female C57BL/6J mice were intraperitoneally (ip) administration with either saline or 200 mg/kg of cyclophosphamide (CYP) 48 h before experimental evaluation. Cystitis mice were treated with administration of gabapentin (25 or 50 mg/kg, ip). Cystometry and EUS EMG were obtained and analyzed during continuous bladder infusion. The visceral pain-related visceromotor reflex (VMR) was recorded in response to isotonic bladder distension. Results: Cystitis mice showed shorter inter-contraction intervals and increased occurrence of non-voiding contractions during bladder infusion, with increased VMR during isotonic bladder distension, indicating cystitis-induced bladder hyperalgesia. Gabapentin (50 mg/kg) suppressed effects of CYP on cystometry, but not on EUS EMG activity, during bladder infusion. The effect on urodynamic recordings lasted 4 h. VMR was significantly reduced by gabapentin. Conclusions: The present study showed that CYP-induced cystitis in mice is a model of visceral hyperalgesia affecting detrusor contractions, not urethral activations. The technique of using EUS EMG to evaluate the drug effects on urethral activities is novel and useful for future investigations. Gabapentin can be as a potential treatment for detrusor overactivity and PBS/IC.

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