Involvement of focal adhesion kinase in hepatocyte growth factor-induced scatter of Madin-Darby canine kidney cells

Jui Fen Lai, Shu Chen Kao, Si Tse Jiang, Ming-Jer Tang, Po Chao Chan, Hong Chen Chen

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Abstract

Focal adhesion kinase (FAK) has been implicated to play a critical role in integrin-mediated control of cell behavior. However, it is unclear whether FAK also participates in the regulation of growth factor-elicited cellular functions. In this study, we have demonstrated that although overexpression of FAK in Madin-Dardy canine kidney cells did not alter their growth property or ability to form tubules within collagen gel upon hepatocyte growth factor (HGF) stimulation, it apparently enhanced HGF-induced cell scattering. This enhancement was largely because of an increase in the third phase (i.e. cell migration) of cell scattering rather than the first two phases (i.e. cell spreading and cell-cell dissociation). Conversely, the expression of FAK- related nonkinase significantly (~60%) inhibited HGF-induced cell migration. Moreover, we have found that the effect of FAK on promoting HGF-induced cell motility was greatly dependent on cell-matrix interactions. We showed that HGF treatment selectively increased the expression of integrins α2 and, to a lesser extent, α3 in Madin-Dardy canine kidney cells and that a monoclonal antibody against integrin α2 efficiently blocked HGF-enhanced cell migration on collagen. In our efforts to determine the mechanism by which FAK promotes HGF-induced cell migration, we found that FAK mutants deficient in phosphatidylinositol 3-kinase or p130(Cas) binding failed to promote HGF-induced cell migration. Interestingly, cells expressing a FAK mutant defective in Grb2 binding exhibited a rate of migration ~50% lower than that of cells expressing wild type FAK in response to HGF stimulation. Taken together, our results suggest a link between HGF-increased integrin expression, FAK activation, and enhanced cell motility and implicate a role for FAK in the facilitation of growth factor-induced cell motility.

Original languageEnglish
Pages (from-to)7474-7480
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number11
DOIs
Publication statusPublished - 2000 Mar 17

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Focal Adhesion Protein-Tyrosine Kinases
Madin Darby Canine Kidney Cells
Hepatocyte Growth Factor
Cell Movement
Integrins
Canidae
Intercellular Signaling Peptides and Proteins
Collagen
Cells
Phosphatidylinositol 3-Kinase
Scattering
Kidney
Behavior Control
Aptitude
Cell Communication
Gels
Chemical activation
Monoclonal Antibodies

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Lai, Jui Fen ; Kao, Shu Chen ; Jiang, Si Tse ; Tang, Ming-Jer ; Chan, Po Chao ; Chen, Hong Chen. / Involvement of focal adhesion kinase in hepatocyte growth factor-induced scatter of Madin-Darby canine kidney cells. In: Journal of Biological Chemistry. 2000 ; Vol. 275, No. 11. pp. 7474-7480.
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abstract = "Focal adhesion kinase (FAK) has been implicated to play a critical role in integrin-mediated control of cell behavior. However, it is unclear whether FAK also participates in the regulation of growth factor-elicited cellular functions. In this study, we have demonstrated that although overexpression of FAK in Madin-Dardy canine kidney cells did not alter their growth property or ability to form tubules within collagen gel upon hepatocyte growth factor (HGF) stimulation, it apparently enhanced HGF-induced cell scattering. This enhancement was largely because of an increase in the third phase (i.e. cell migration) of cell scattering rather than the first two phases (i.e. cell spreading and cell-cell dissociation). Conversely, the expression of FAK- related nonkinase significantly (~60{\%}) inhibited HGF-induced cell migration. Moreover, we have found that the effect of FAK on promoting HGF-induced cell motility was greatly dependent on cell-matrix interactions. We showed that HGF treatment selectively increased the expression of integrins α2 and, to a lesser extent, α3 in Madin-Dardy canine kidney cells and that a monoclonal antibody against integrin α2 efficiently blocked HGF-enhanced cell migration on collagen. In our efforts to determine the mechanism by which FAK promotes HGF-induced cell migration, we found that FAK mutants deficient in phosphatidylinositol 3-kinase or p130(Cas) binding failed to promote HGF-induced cell migration. Interestingly, cells expressing a FAK mutant defective in Grb2 binding exhibited a rate of migration ~50{\%} lower than that of cells expressing wild type FAK in response to HGF stimulation. Taken together, our results suggest a link between HGF-increased integrin expression, FAK activation, and enhanced cell motility and implicate a role for FAK in the facilitation of growth factor-induced cell motility.",
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Involvement of focal adhesion kinase in hepatocyte growth factor-induced scatter of Madin-Darby canine kidney cells. / Lai, Jui Fen; Kao, Shu Chen; Jiang, Si Tse; Tang, Ming-Jer; Chan, Po Chao; Chen, Hong Chen.

In: Journal of Biological Chemistry, Vol. 275, No. 11, 17.03.2000, p. 7474-7480.

Research output: Contribution to journalArticle

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AU - Kao, Shu Chen

AU - Jiang, Si Tse

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