Involvement of serine proteinase in infectious pancreatic necrosis virus capsid protein maturation and NS proteinase cleavage in CHSE-214 cells

J. L. Wu, J. R. Hong, C. Y. Chang, C. F. Hui, C. F. Liao, Y. L. Hsu

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

An investigation of virus-specific protein maturation in infectious pancreatic necrosis virus (IPNV) infected Chinook salmon embryo cells (CHSE-214) was undertaken. The precursor protein (pVP2-1) of the major mature capsid protein (VP2) was processed sequentially from pVP2-1 to pVP2-2 and VP2. Experiments using serine proteinase inhibitors showed that the maturation of the VP2 was blocked in the pVP2-1 post-translational cleavage steps. A protinin, a potent proteinase inhibitor, at 800 μg ml-1 blocked pVP2-2 to VP2 and the cleavage of VP4 (28 kDa) to VP4-1 (25 kDa). Therefore, our data showed that the maturation of the capsid protein (VP2) and cleavage of VP4 (NS proteinase) can be blocked by serine proteinase inhibitors.

Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalJournal of Fish Diseases
Volume21
Issue number3
DOIs
Publication statusPublished - 1998 May

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • veterinary (miscalleneous)

Fingerprint Dive into the research topics of 'Involvement of serine proteinase in infectious pancreatic necrosis virus capsid protein maturation and NS proteinase cleavage in CHSE-214 cells'. Together they form a unique fingerprint.

Cite this