Involvement of TG-interacting factor in microglial activation during experimental traumatic brain injury

Chung Ching Chio, Ching Ping Chang, Mao Tsun Lin, Fang Cheng Su, Chung Zhing Yang, Hong Yu Tseng, Zi Miao Liu, Huei-Sheng Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Traumatic brain injury (TBI) is a complex injury involving several physiological alterations, potentially leading to neurological impairment. Previous mouse studies using high-density oligonucleotide array analysis have confirmed the upregulation of transforming growth-interacting factor (TGIF) mRNA in TBI. TGIF is a transcriptional corepressor of transforming growth factor beta (TGF-β) signaling which plays a protective role in TBI. However, the functional roles of TGIF in TBI are not well understood. In this study, we used confocal microscopy after immunofluorescence staining to demonstrate the increase of TGIF levels in the activated microglia of the pericontusional cortex of rats with TBI. Intracerebral knockdown of TGIF in the pericontusional cortex significantly downregulated TGIF expression, attenuated microglial activation, reduced the volume of damaged brain tissue, and facilitated recovery of limb motor function. Collectively, our results indicate that TGIF is involved in TBI-induced microglial activation, resulting in secondary brain injury and motor dysfunction.

Original languageEnglish
Pages (from-to)816-824
Number of pages9
JournalJournal of Neurochemistry
Volume131
Issue number6
DOIs
Publication statusPublished - 2015 Jan 1

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Transforming Growth Factors
Brain
Chemical activation
Co-Repressor Proteins
Microglia
Traumatic Brain Injury
Oligonucleotide Array Sequence Analysis
Confocal microscopy
Confocal Microscopy
Transforming Growth Factor beta
Brain Injuries
Fluorescent Antibody Technique
Intercellular Signaling Peptides and Proteins
Oligonucleotides
Up-Regulation
Down-Regulation
Extremities
Rats
Staining and Labeling
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Chio, Chung Ching ; Chang, Ching Ping ; Lin, Mao Tsun ; Su, Fang Cheng ; Yang, Chung Zhing ; Tseng, Hong Yu ; Liu, Zi Miao ; Huang, Huei-Sheng. / Involvement of TG-interacting factor in microglial activation during experimental traumatic brain injury. In: Journal of Neurochemistry. 2015 ; Vol. 131, No. 6. pp. 816-824.
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Involvement of TG-interacting factor in microglial activation during experimental traumatic brain injury. / Chio, Chung Ching; Chang, Ching Ping; Lin, Mao Tsun; Su, Fang Cheng; Yang, Chung Zhing; Tseng, Hong Yu; Liu, Zi Miao; Huang, Huei-Sheng.

In: Journal of Neurochemistry, Vol. 131, No. 6, 01.01.2015, p. 816-824.

Research output: Contribution to journalArticle

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