Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation

Tomotake Ote, Masayuki Hashimoto, Yoshiho Ikeuchi, Masayuki Su'etsugu, Tsutomu Suzuki, Tsutomu Katayama, Jun Ichi Kato

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The Escherichia coli hda gene codes for a DnaA-related protein that is essential for the regulatory inactivation of DnaA (RIDA), a system that controls the initiation of chromosomal replication. We have identified the ygfZ gene, which encodes a folate-binding protein, as a suppressor of hda mutations. The ygfZ null mutation suppresses an hda null mutation. The over-initiation and abortive elongation phenotypes conferred by the hda mutations are partially suppressed in an hda ygfZ background. The accumulation of the active form of DnaA, ATP-DnaA, in the hda mutant is suppressed by the disruption of the ygfZ gene, indicating that YgfZ is involved in regulating the level of ATP-DnaA. Although ygfZ is not an essential gene, the ygfZ disruptant grows slowly, especially at low temperature, demonstrating that this gene is important for cellular proliferation. We have identified mnmE (trmE) as a suppressor of the ygfZ disruption. This gene encodes a GTPase involved in tRNA modification. Examination of RNA modification in the ygfZ mutant reveals reduced levels of 2-methylthio-6-iodeadenosine, indicating that YgfZ participates in the methylthio-group formation of this modified nucleoside in some tRNAs. These results suggest that YgfZ is a key factor in regulatory networks that act via tRNA modification.

Original languageEnglish
Pages (from-to)265-275
Number of pages11
JournalMolecular Microbiology
Volume59
Issue number1
DOIs
Publication statusPublished - 2006 Jan 1

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Folic Acid
Carrier Proteins
RNA
Escherichia coli
Transfer RNA
Genes
Mutation
Adenosine Triphosphate
Genetic Suppression
GTP Phosphohydrolases
Essential Genes
Nucleosides
Cell Proliferation
Phenotype
Temperature
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Ote, Tomotake ; Hashimoto, Masayuki ; Ikeuchi, Yoshiho ; Su'etsugu, Masayuki ; Suzuki, Tsutomu ; Katayama, Tsutomu ; Kato, Jun Ichi. / Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation. In: Molecular Microbiology. 2006 ; Vol. 59, No. 1. pp. 265-275.
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Involvement of the Escherichia coli folate-binding protein YgfZ in RNA modification and regulation of chromosomal replication initiation. / Ote, Tomotake; Hashimoto, Masayuki; Ikeuchi, Yoshiho; Su'etsugu, Masayuki; Suzuki, Tsutomu; Katayama, Tsutomu; Kato, Jun Ichi.

In: Molecular Microbiology, Vol. 59, No. 1, 01.01.2006, p. 265-275.

Research output: Contribution to journalArticle

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