Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA)

Cheng Yu Hung, Kuo Hsiung Wang, Chi Chun Huang, Xun Gong, Xue Jun Ge, Tzen Yuh Chiang

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

We report 11 novel microsatellite primer pairs for the wild tea, Camellia sinensis (L.) O. Kuntze forma formosensis Kitamura. These simple sequence repeat markers were tested in 24 samples collected from wild tea populations, and in cultivars and C. japonica. The number of alleles ranged from 4 to18. The expected (H E) and observed (H O) heterozygosity were 0.687-0.946 and 0.042-0.792, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the rare species. Significant LD was discovered in most loci. These primers may provide a tool for understanding demography and population structure in wild tea.

Original languageEnglish
Pages (from-to)779-781
Number of pages3
JournalConservation Genetics
Volume9
Issue number3
DOIs
Publication statusPublished - 2008 Jun

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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