TY - JOUR
T1 - Isolation and characterization of 15 microsatellite loci from Caridina gracilipes (Atyidae, Decapoda)
AU - Han, Chiao Chuan
AU - Chang, Cheng Sheng
AU - Chiang, Tzen-Yuh
AU - Chung, Ping Han
AU - Lin, Hung Du
PY - 2009/8/1
Y1 - 2009/8/1
N2 - Caridina gracilipes, an atyid shrimp of Taiwan, inhabits the fresh and brackish waters. Its life history can be classified into amphidromous and landlocked types. Recent human's transportation has caused concerns of genetic contamination between these long evolved lineages and ecological instabilities. Molecular markers with high sensitivities are required for detecting the possible introgression due to human disturbance. In the study, we described the development of 15 polymorphic microsatellite loci in C. gracilipes for genetic and ecological studies. These new markers were tested in 20 individuals of three populations. The number of alleles ranged from 6 to 17. Expected (H E) and observed (H O) heterozygosities ranged from 0.653 to 0.954, averaged at 0.865, and from 0.000 to 0.4, averaged at 0.146, respectively. All loci are significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency. Significant genetic differentiation among populations was detected based on F ST values, which were estimated from 0.82 to 0.88.
AB - Caridina gracilipes, an atyid shrimp of Taiwan, inhabits the fresh and brackish waters. Its life history can be classified into amphidromous and landlocked types. Recent human's transportation has caused concerns of genetic contamination between these long evolved lineages and ecological instabilities. Molecular markers with high sensitivities are required for detecting the possible introgression due to human disturbance. In the study, we described the development of 15 polymorphic microsatellite loci in C. gracilipes for genetic and ecological studies. These new markers were tested in 20 individuals of three populations. The number of alleles ranged from 6 to 17. Expected (H E) and observed (H O) heterozygosities ranged from 0.653 to 0.954, averaged at 0.865, and from 0.000 to 0.4, averaged at 0.146, respectively. All loci are significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency. Significant genetic differentiation among populations was detected based on F ST values, which were estimated from 0.82 to 0.88.
UR - https://www.scopus.com/pages/publications/67649383298
UR - https://www.scopus.com/pages/publications/67649383298#tab=citedBy
U2 - 10.1007/s10592-008-9689-8
DO - 10.1007/s10592-008-9689-8
M3 - Article
AN - SCOPUS:67649383298
SN - 1566-0621
VL - 10
SP - 1065
EP - 1068
JO - Conservation Genetics
JF - Conservation Genetics
IS - 4
ER -