Isolation and characterization of a Vibrio vulnificus mutant deficient in both extracellular metalloprotease and cytolysin

J. J. Fan, C. P. Shao, Y. C. Ho, Chun-Keung Yu, L. I. Hor

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

We isolated a Vibrio vulnificus mutant that was deficient in both metalloprotease and cytolysin by allelic exchange. The virulence of this mutant in mice and its cytotoxicity for HEp-2 cells were comparable to those of the wild-type strain, indicating that neither factor was essential for these properties. The cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice.

Original languageEnglish
Pages (from-to)5943-5948
Number of pages6
JournalInfection and Immunity
Volume69
Issue number9
DOIs
Publication statusPublished - 2001 Sep 6

Fingerprint

Vibrio vulnificus
Perforin
Metalloproteases
Virulence
Peptide Hydrolases

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

@article{0f07309148c24aecb84e5aa27b2280c9,
title = "Isolation and characterization of a Vibrio vulnificus mutant deficient in both extracellular metalloprotease and cytolysin",
abstract = "We isolated a Vibrio vulnificus mutant that was deficient in both metalloprotease and cytolysin by allelic exchange. The virulence of this mutant in mice and its cytotoxicity for HEp-2 cells were comparable to those of the wild-type strain, indicating that neither factor was essential for these properties. The cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice.",
author = "Fan, {J. J.} and Shao, {C. P.} and Ho, {Y. C.} and Chun-Keung Yu and Hor, {L. I.}",
year = "2001",
month = "9",
day = "6",
doi = "10.1128/IAI.69.9.5943-5948.2001",
language = "English",
volume = "69",
pages = "5943--5948",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "9",

}

Isolation and characterization of a Vibrio vulnificus mutant deficient in both extracellular metalloprotease and cytolysin. / Fan, J. J.; Shao, C. P.; Ho, Y. C.; Yu, Chun-Keung; Hor, L. I.

In: Infection and Immunity, Vol. 69, No. 9, 06.09.2001, p. 5943-5948.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation and characterization of a Vibrio vulnificus mutant deficient in both extracellular metalloprotease and cytolysin

AU - Fan, J. J.

AU - Shao, C. P.

AU - Ho, Y. C.

AU - Yu, Chun-Keung

AU - Hor, L. I.

PY - 2001/9/6

Y1 - 2001/9/6

N2 - We isolated a Vibrio vulnificus mutant that was deficient in both metalloprotease and cytolysin by allelic exchange. The virulence of this mutant in mice and its cytotoxicity for HEp-2 cells were comparable to those of the wild-type strain, indicating that neither factor was essential for these properties. The cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice.

AB - We isolated a Vibrio vulnificus mutant that was deficient in both metalloprotease and cytolysin by allelic exchange. The virulence of this mutant in mice and its cytotoxicity for HEp-2 cells were comparable to those of the wild-type strain, indicating that neither factor was essential for these properties. The cytolysin, but not the protease, seemed to be important for causing damage in the alimentary tract of the mice.

UR - http://www.scopus.com/inward/record.url?scp=0034865707&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034865707&partnerID=8YFLogxK

U2 - 10.1128/IAI.69.9.5943-5948.2001

DO - 10.1128/IAI.69.9.5943-5948.2001

M3 - Article

VL - 69

SP - 5943

EP - 5948

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 9

ER -