Isolation and characterization of Chlorella sp. mutants with enhanced thermo- And CO2 tolerances for CO2 sequestration and utilization of flue gases

Hsiang Hui Chou, Hsiang Yen Su, Xiang Di Song, Te Jin Chow, Chun Yen Chen, Jo Shu Chang, Tse Min Lee

Research output: Contribution to journalArticle

Abstract

Background: The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results: In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80-90 ppm SO2, 90-100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions: The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.

Original languageEnglish
Article number251
JournalBiotechnology for Biofuels
Volume12
Issue number1
DOIs
Publication statusPublished - 2019 Oct 19

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Chlorella
Flue gases
carbon sequestration
tolerance
Gases
Chlorella vulgaris
Carbohydrates
Temperature
carbohydrate
mitigation
Global Warming
Microalgae
Methylnitronitrosoguanidine
Mutagenesis
Environmental Pollution
Biofuels
Climate Change
Fasteners
Global warming
Biodiesel

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Energy(all)
  • Management, Monitoring, Policy and Law

Cite this

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title = "Isolation and characterization of Chlorella sp. mutants with enhanced thermo- And CO2 tolerances for CO2 sequestration and utilization of flue gases",
abstract = "Background: The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results: In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25{\%} CO2, 80-90 ppm SO2, 90-100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions: The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.",
author = "Chou, {Hsiang Hui} and Su, {Hsiang Yen} and Song, {Xiang Di} and Chow, {Te Jin} and Chen, {Chun Yen} and Chang, {Jo Shu} and Lee, {Tse Min}",
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Isolation and characterization of Chlorella sp. mutants with enhanced thermo- And CO2 tolerances for CO2 sequestration and utilization of flue gases. / Chou, Hsiang Hui; Su, Hsiang Yen; Song, Xiang Di; Chow, Te Jin; Chen, Chun Yen; Chang, Jo Shu; Lee, Tse Min.

In: Biotechnology for Biofuels, Vol. 12, No. 1, 251, 19.10.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Isolation and characterization of Chlorella sp. mutants with enhanced thermo- And CO2 tolerances for CO2 sequestration and utilization of flue gases

AU - Chou, Hsiang Hui

AU - Su, Hsiang Yen

AU - Song, Xiang Di

AU - Chow, Te Jin

AU - Chen, Chun Yen

AU - Chang, Jo Shu

AU - Lee, Tse Min

PY - 2019/10/19

Y1 - 2019/10/19

N2 - Background: The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results: In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80-90 ppm SO2, 90-100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions: The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.

AB - Background: The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results: In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80-90 ppm SO2, 90-100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions: The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.

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U2 - 10.1186/s13068-019-1590-9

DO - 10.1186/s13068-019-1590-9

M3 - Article

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VL - 12

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