Abstract
Background: The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results: In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80-90 ppm SO2, 90-100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions: The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.
| Original language | English |
|---|---|
| Article number | 251 |
| Journal | Biotechnology for Biofuels |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2019 Oct 19 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
- Renewable Energy, Sustainability and the Environment
- Energy(all)
- Management, Monitoring, Policy and Law
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Isolation and characterization of Chlorella sp. mutants with enhanced thermo- And CO2 tolerances for CO2 sequestration and utilization of flue gases. / Chou, Hsiang Hui; Su, Hsiang Yen; Song, Xiang Di; Chow, Te Jin; Chen, Chun Yen; Chang, Jo Shu; Lee, Tse Min.
In: Biotechnology for Biofuels, Vol. 12, No. 1, 251, 19.10.2019.Research output: Contribution to journal › Article
TY - JOUR
T1 - Isolation and characterization of Chlorella sp. mutants with enhanced thermo- And CO2 tolerances for CO2 sequestration and utilization of flue gases
AU - Chou, Hsiang Hui
AU - Su, Hsiang Yen
AU - Song, Xiang Di
AU - Chow, Te Jin
AU - Chen, Chun Yen
AU - Chang, Jo Shu
AU - Lee, Tse Min
PY - 2019/10/19
Y1 - 2019/10/19
N2 - Background: The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results: In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80-90 ppm SO2, 90-100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions: The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.
AB - Background: The increasing emission of flue gas from industrial plants contributes to environmental pollution, global warming, and climate change. Microalgae have been considered excellent biological materials for flue gas removal, particularly CO2 mitigation. However, tolerance to high temperatures is also critical for outdoor microalgal mass cultivation. Therefore, flue gas- and thermo-tolerant mutants of Chlorella vulgaris ESP-31 were generated and characterized for their ability to grow under various conditions. Results: In this study, we obtained two CO2- and thermo-tolerant mutants of Chlorella vulgaris ESP-31, namely, 283 and 359, with enhanced CO2 tolerance and thermo-tolerance by using N-methyl-N-nitro-N-nitrosoguanidine (NTG) mutagenesis followed by screening at high temperature and under high CO2 conditions with the w-zipper pouch selection method. The two mutants exhibited higher photosynthetic activity and biomass productivity than that of the ESP-31 wild type. More importantly, the mutants were able to grow at high temperature (40 °C) and a high concentration of simulated flue gas (25% CO2, 80-90 ppm SO2, 90-100 ppm NO) and showed higher carbohydrate and lipid contents than did the ESP-31 wild type. Conclusions: The two thermo- and flue gas-tolerant mutants of Chlorella vulgaris ESP-31 were useful for CO2 mitigation from flue gas under heated conditions and for the production of carbohydrates and biodiesel directly using CO2 from flue gas.
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U2 - 10.1186/s13068-019-1590-9
DO - 10.1186/s13068-019-1590-9
M3 - Article
AN - SCOPUS:85074233418
VL - 12
JO - Biotechnology for Biofuels
JF - Biotechnology for Biofuels
SN - 1754-6834
IS - 1
M1 - 251
ER -