Isolation and characterization of microsatellite loci from a potential biofuel plant Miscanthus sinensis (Poaceae)

Kuo Hsiang Hung, Tzen Yuh Chiang, Chi Te Chiu, Tsai Wen Hsu, Chuan Wen Ho

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31 Citations (Scopus)

Abstract

Miscanthus sinensis (Poaceae) is typical of many dominant grasses of East Asia. Due to its characteristics of fast growth and high biomass, Miscanthus, a C4 plant, has been long explored for the potential usage as biofuel. In this study, we described the development of nine microsatellite loci from M. sinensis for genetic studies. These new markers were tested in 25 individuals of five populations in three varieties. The number of alleles ranged from 3 to 8. The expected (HE) and observed (HO) heterozygosities were 0.37-0.78 and 0.05-0.52, respectively. All microsatellite loci are significantly deviated from Hardy-Weinberg expectations likely due to the population structure within samples. Linkage disequilibrium between four loci pairs were observed from the total 36 pairwise comparisons of loci. Cross-species transferability revealed that all loci can be applied to its closely related species, M. floridulus.

Original languageEnglish
Pages (from-to)1377-1380
Number of pages4
JournalConservation Genetics
Volume10
Issue number5
DOIs
Publication statusPublished - 2009 Sep

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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