Isolation and characterization of microsatellite loci from Pinus massoniana (pinaceae)

Kuo Hsiang Hung, Chi Yung Lin, Chi Chun Huang, Chi Chuan Hwang, Tsai Wen Hsu, Yau Lun Kuo, Wei Kuang Wang, Cheng Yu Hung, Tzen Yuh Chiang

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8 Citations (Scopus)


Pinus massoniana is widespread in the central and eastern mainland China, while distributed in Taiwan with a single population. This island population has been declining due to habitat destruction and pine wilt disease. In the study we isolated microsatellite loci from P. massoniana for investigating the population structure. Eleven, novel microsatellite markers were developed from P. massoniana by using a modified PCR-based isolation of microsatellite arrays (PIMA) method. The number of alleles, observed and expected heterozygosities across loci varied with a range of 2-9, 0.00-0.82 and 0.40-0.83, respectively. The application of these microsatellite markers of P. massoniana provides a tool for understanding demography and population structure in Taiwan and mainland China.

Original languageEnglish
Pages (from-to)191-196
Number of pages6
JournalBotanical Studies
Issue number2
Publication statusPublished - 2012 Apr 1

All Science Journal Classification (ASJC) codes

  • Plant Science


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