Isolation and characterization of microsatellite loci in Pedicularis verticillata L. using PCR-based isolation of microsatellite arrays (PIMA)

Tsurng Juhn Huang, Yi Yen Chen, Yen Ping Li, Cheng Yu Hung, Tzen-Yuh Chiang, Chang Hung Chou

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Pedicularis verticillata L. is a highly valuable herb for traditional Chinese medical treatment. In this report, 11 microsatellite loci from P. verticillata were isolated. The simple sequence repeat (SSR) markers were screened in 23 samples of wild populations of P. verticillata, and eight samples from its sister P. ikomai. The number of alleles ranged from 4 to 13, and value of expected (HE) and observed (H0) heterozygosity was 0.62609-0.89662 and 0-0.95652, respectively. All loci were significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency, indicating a dramatic loss of genetic polymorphisms in the restrictedly distributed species. The markers amplified well in the two species are useful for examining genetic diversity and population genetic structure, which, in turn, can provide information for establishing conservation strategy for these endangered species.

Original languageEnglish
Pages (from-to)1389-1391
Number of pages3
JournalConservation Genetics
Volume9
Issue number5
DOIs
Publication statusPublished - 2008 Oct 1

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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