Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA)

Chi Chun Huang; Tzen Yuh Chiang; Tsai Wen Hsu

doi:10.1007/s10592-007-9341-z

Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA)

Chi Chun Huang, Tzen Yuh Chiang, Tsai Wen Hsu

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Taxus sumatrana (Miq.) de Laub. (Taxaceae) is an endangered conifer with a scattered distribution in central Taiwan. In this study, we described the development of 12 microsatellite loci in T. sumatrana for genetic studies. These new markers were tested in nine individuals of the rare species. The number of alleles ranged from 3 to 13 and expected heterozygosity from 0.627 to 0.948. Eleven of twelve loci are significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency.

Original language	English
Pages (from-to)	471-473
Number of pages	3
Journal	Conservation Genetics
Volume	9
Issue number	2
DOIs	https://doi.org/10.1007/s10592-007-9341-z
Publication status	Published - 2008 Apr

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Genetics

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AU - Hsu, Tsai Wen

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AB - Taxus sumatrana (Miq.) de Laub. (Taxaceae) is an endangered conifer with a scattered distribution in central Taiwan. In this study, we described the development of 12 microsatellite loci in T. sumatrana for genetic studies. These new markers were tested in nine individuals of the rare species. The number of alleles ranged from 3 to 13 and expected heterozygosity from 0.627 to 0.948. Eleven of twelve loci are significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency.

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Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA)

Abstract

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