Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA)

Chi Chun Huang, Tzen Yuh Chiang, Tsai Wen Hsu

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Taxus sumatrana (Miq.) de Laub. (Taxaceae) is an endangered conifer with a scattered distribution in central Taiwan. In this study, we described the development of 12 microsatellite loci in T. sumatrana for genetic studies. These new markers were tested in nine individuals of the rare species. The number of alleles ranged from 3 to 13 and expected heterozygosity from 0.627 to 0.948. Eleven of twelve loci are significantly deviated from Hardy-Weinberg expectations due to the heterozygote deficiency.

Original languageEnglish
Pages (from-to)471-473
Number of pages3
JournalConservation Genetics
Volume9
Issue number2
DOIs
Publication statusPublished - 2008 Apr 1

Fingerprint

Taxaceae
Taxus
Coniferophyta
Heterozygote
rare species
Taiwan
heterozygosity
Microsatellite Repeats
coniferous tree
allele
Alleles
microsatellite repeats
Polymerase Chain Reaction
loci
conifers
alleles
Taxus sumatrana
distribution
marker

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

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Isolation and characterization of microsatellite loci in Taxus sumatrana (Taxaceae) using PCR-based isolation of microsatellite arrays (PIMA). / Huang, Chi Chun; Chiang, Tzen Yuh; Hsu, Tsai Wen.

In: Conservation Genetics, Vol. 9, No. 2, 01.04.2008, p. 471-473.

Research output: Contribution to journalArticle

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