Isolation and characterization of polymorphic microsatellite loci from Areca catechu (Arecaceae) using PCR-based isolation of microsatellite arrays

Cheng Heng Hu, Chi Chun Huang, Kuo Hsiang Hung, Tsai Wen Hsu, Tzen Yuh Chiang

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1 Citation (Scopus)

Abstract

Betel nut (Areca nut, Areca catechu L.) is a conspicuous and important cultivated plant of tropical and subtropical habitats throughout Southeast Asia and Oceania. As a significant cultural and social offering, the migration of betel nut associated with human dispersal is an important issue in ethnobotany and anthropology. In this study, we described the development of nine microsatellite loci from A. catechu. The number of alleles per locus ranged from 5 to 15. The expected and observed heterozygosities ranged from 0.71to 0.94 and from 0 to 0.88, respectively. All microsatellite loci, except for AC30, significantly deviated from Hardy-Weinberg equilibrium possibly due to artificially selected cultivation or the existence of excessive null alleles. No linkage disequilibrium was observed from pairwise comparisons of loci, except for AC06 and AC08.

Original languageEnglish
Pages (from-to)658-660
Number of pages3
JournalMolecular Ecology Resources
Volume9
Issue number2
DOIs
Publication statusPublished - 2009 Mar 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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