Isolation and characterization of polymorphic microsatellite loci in Hemibarbus labeo (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)

Chung Jian Lin, Jiang Ping Wang, Hung Du Lin, Tzen-Yuh Chiang

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12 Citations (Scopus)

Abstract

A total of 10 polymorphic microsatellite loci from Hemibarbus labeo were isolated and characterized using an optimized protocol to construct a microsatellite-enriched genomic library. The analysis of variability was performed in 24 specimens of mainland China. The mean number of alleles across loci was 3.10 ± 1.10 and the level of expected heterozygosity varied from 0.0417 to 0.7482. Frequencies of null alleles of the 10 loci are not significantly greater than zero. No linkage disequilibrium was detected between loci in either population. Five primer pairs cross-amplify the microsatellites in other species, indicating transportability of the markers within the family Cyprinidae.

Original languageEnglish
Pages (from-to)788-790
Number of pages3
JournalMolecular Ecology Notes
Volume7
Issue number5
DOIs
Publication statusPublished - 2007 Sep 1

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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