Isolation and characterization of polymorphic microsatellite loci in Hemibarbus labeo (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)

Chung Jian Lin; Jiang Ping Wang; Hung Du Lin; Tzen Yuh Chiang

doi:10.1111/j.1471-8286.2007.01702.x

Isolation and characterization of polymorphic microsatellite loci in Hemibarbus labeo (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)

Chung Jian Lin, Jiang Ping Wang, Hung Du Lin, Tzen Yuh Chiang

Department of Life Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

A total of 10 polymorphic microsatellite loci from Hemibarbus labeo were isolated and characterized using an optimized protocol to construct a microsatellite-enriched genomic library. The analysis of variability was performed in 24 specimens of mainland China. The mean number of alleles across loci was 3.10 ± 1.10 and the level of expected heterozygosity varied from 0.0417 to 0.7482. Frequencies of null alleles of the 10 loci are not significantly greater than zero. No linkage disequilibrium was detected between loci in either population. Five primer pairs cross-amplify the microsatellites in other species, indicating transportability of the markers within the family Cyprinidae.

Original language	English
Pages (from-to)	788-790
Number of pages	3
Journal	Molecular Ecology Notes
Volume	7
Issue number	5
DOIs	https://doi.org/10.1111/j.1471-8286.2007.01702.x
Publication status	Published - 2007 Sept

All Science Journal Classification (ASJC) codes

Biochemistry
Ecology
General Biochemistry,Genetics and Molecular Biology

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10.1111/j.1471-8286.2007.01702.x

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title = "Isolation and characterization of polymorphic microsatellite loci in Hemibarbus labeo (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)",

abstract = "A total of 10 polymorphic microsatellite loci from Hemibarbus labeo were isolated and characterized using an optimized protocol to construct a microsatellite-enriched genomic library. The analysis of variability was performed in 24 specimens of mainland China. The mean number of alleles across loci was 3.10 ± 1.10 and the level of expected heterozygosity varied from 0.0417 to 0.7482. Frequencies of null alleles of the 10 loci are not significantly greater than zero. No linkage disequilibrium was detected between loci in either population. Five primer pairs cross-amplify the microsatellites in other species, indicating transportability of the markers within the family Cyprinidae.",

author = "Lin, {Chung Jian} and Wang, {Jiang Ping} and Lin, {Hung Du} and Chiang, {Tzen Yuh}",

year = "2007",

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AU - Lin, Chung Jian

AU - Wang, Jiang Ping

AU - Lin, Hung Du

AU - Chiang, Tzen Yuh

PY - 2007/9

Y1 - 2007/9

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AB - A total of 10 polymorphic microsatellite loci from Hemibarbus labeo were isolated and characterized using an optimized protocol to construct a microsatellite-enriched genomic library. The analysis of variability was performed in 24 specimens of mainland China. The mean number of alleles across loci was 3.10 ± 1.10 and the level of expected heterozygosity varied from 0.0417 to 0.7482. Frequencies of null alleles of the 10 loci are not significantly greater than zero. No linkage disequilibrium was detected between loci in either population. Five primer pairs cross-amplify the microsatellites in other species, indicating transportability of the markers within the family Cyprinidae.

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Isolation and characterization of polymorphic microsatellite loci in Hemibarbus labeo (Cyprinidae) using PCR-based isolation of microsatellite arrays (PIMA)

Abstract

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