Isolation and characterization of polymorphic microsatellite loci from metapenaeopsis barbata using PCR-based isolation of microsatellite arrays (PIMA)

Tzen Yuh Chiang, Tzong Der Tzeng, Hung Du Lin, Ching Ju Cho, Feng Jiau Lin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The red-spot prawn, Metapenaeopsis barbata, is a commercially important, widely distributed demersal species in the Indo-West Pacific Ocean. Over fishing has made its populations decline in the past decade. To study conservation genetics, eight polymorphic microsatellite loci were isolated. Genetic characteristics of the SSR (simple sequence repeat) fingerprints were estimated in 61 individuals from adjacent seas of Taiwan and China. The number of alleles, ranging from 2 to 4, as well as observed and expected heterozygosities in populations, ranging from 0.048 to 0.538, and 0.048 and 0.654, respectively, were detected. No deviation from Hardy-Weinberg expectations was detected at either locus. No significant linkage disequilibrium was detected in locus pairs. The polymorphic microsatellite loci will be useful for investigations of the genetic variation, population structure, and conservation genetics of this species.

Original languageEnglish
Pages (from-to)2763-2768
Number of pages6
JournalInternational journal of molecular sciences
Volume13
Issue number3
DOIs
Publication statusPublished - 2012 Mar 1

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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