Isolation and characterization of the human interleukin-9 receptor gene

Ming-Shi Chang, Greg Engel, Chris Benedict, Rita Basu, Jennifer McNinch

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

To better understand the regulation of interleukin-9 (IL-9) receptor expression, we have isolated the genomic clone of the human IL-9 receptor based on its sequence homology with a human IL-9 receptor cDNA isolated from the human megakaryocyte cell line UT-7. The entire genomic structure has been determined. The human IL-9 receptor gene consists of 10 exons spread over approximately 13.7 kb of DNA. The nucleotide sequence of the coding region from the genomic DNA is identical to our cDNA clone. Several blocks of transcriptional control sequence have been identified at the 5' noncoding region of the IL-9 receptor gene that may play an important role in the regulation of the IL-9 receptor gene. A fusion gene containing 659 bp of human IL-9 receptor 5' noncoding region linked to the firefly luciferase gene directed expression of luciferase activity in human embryonic kidney 293 cell line, but not in the mouse fibroblast cell line NIH3T3 cells.

Original languageEnglish
Pages (from-to)3199-3205
Number of pages7
JournalBlood
Volume83
Issue number11
Publication statusPublished - 1994 Jun 1

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Interleukin-9 Receptors
Genes
Cells
Complementary DNA
Clone Cells
Firefly Luciferases
Cell Line
Megakaryocytes
HEK293 Cells
Gene Fusion
DNA
Sequence Homology
Luciferases
Exons
Fibroblasts
Gene Expression
Fusion reactions
Nucleotides
human IL9R protein

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Chang, M-S., Engel, G., Benedict, C., Basu, R., & McNinch, J. (1994). Isolation and characterization of the human interleukin-9 receptor gene. Blood, 83(11), 3199-3205.
Chang, Ming-Shi ; Engel, Greg ; Benedict, Chris ; Basu, Rita ; McNinch, Jennifer. / Isolation and characterization of the human interleukin-9 receptor gene. In: Blood. 1994 ; Vol. 83, No. 11. pp. 3199-3205.
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Chang, M-S, Engel, G, Benedict, C, Basu, R & McNinch, J 1994, 'Isolation and characterization of the human interleukin-9 receptor gene', Blood, vol. 83, no. 11, pp. 3199-3205.

Isolation and characterization of the human interleukin-9 receptor gene. / Chang, Ming-Shi; Engel, Greg; Benedict, Chris; Basu, Rita; McNinch, Jennifer.

In: Blood, Vol. 83, No. 11, 01.06.1994, p. 3199-3205.

Research output: Contribution to journalArticle

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Chang M-S, Engel G, Benedict C, Basu R, McNinch J. Isolation and characterization of the human interleukin-9 receptor gene. Blood. 1994 Jun 1;83(11):3199-3205.