Isolation and Identification of a Novel Antioxidant with Antitumour Activity from Serratia ureilytica Using Squid Pen as Fermentation Substrate

Yao Haur Kuo, Tzu-Wen Liang, Kao Cheng Liu, Ya Wen Hsu, Hsiu Ching Hsu, San Lang Wang

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

The antioxidant activity of the culture supernatant of Serratia ureilytica TKU013 with squid pen as the sole carbon/nitrogen source was assessed by three methods, and the phenolic contents were assayed. The supernatant with the highest antioxidant activity was further purified by liquid-liquid partition, revealing the ethyl acetate extract exhibited the strongest antioxidant activity and the highest total phenolic content. Eight fractions were retrieved from silica gel column chromatography of this extract, designated F1-F8. F4 was found to possess the strong antioxidative activity and the highest total phenolic content and also exhibited strong cytotoxic activities against two different tumoural cell lines. A new compound (Serranticin) with antioxidant and antitumor activity was obtained from F4. The structure of Serranticin is analogous to that of siderophores (hexacoordinated catecholamine), which are iron chelators. As such, Serranticin has the potential for use as a deferration agent in various iron overload diseases.

Original languageEnglish
Pages (from-to)451-461
Number of pages11
JournalMarine Biotechnology
Volume13
Issue number3
DOIs
Publication statusPublished - 2011 Jun 1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Aquatic Science

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