TY - JOUR
T1 - Isolation and purification of brown algae fucoidan from Sargassum siliquosum and the analysis of anti-lipogenesis activity
AU - Wang, Shao Hua
AU - Huang, Chih Yu
AU - Chen, Chun Yen
AU - Chang, Chia Che
AU - Huang, Chun Yung
AU - Dong, Cheng Di
AU - Chang, Jo Shu
N1 - Funding Information:
The financial support from the Ministry of Science and Technology, Taiwan , under grant number MOST 107-2221-E-006-112-MY3 , 108-2621-M-006-020- , and 109-3116-F-006-016-CC1 , is highly appreciated.
Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/1/15
Y1 - 2021/1/15
N2 - Fucoidans are sulfated polysaccharides of marine origin and they have distinguished biological applications due to their anti-viral, anti-inflammatory, anti-angiogenic, and anti-tumorigenic potential. In this study, fucoidan was extracted from a brown macroalga (Sargassum siliquosum) using microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). MAE under the condition of 750 W, 10 min, and a liquid/solid ratio of 15 mL/g, showed higher extraction efficiency with a total sugar yield of 6.94% per dry weight. The crude fucoidan obtained with ethanol precipitation was further purified by isoelectric point precipitation and calcium chloride addition (for protein and uronic acid removal), followed by anion-exchange chromatography (AEC) and dialysis. The purity of the polysaccharides content of crude fucoidan increased from 33.3–64.5% with a total sugar recovery of 50.3%. Based on the sugar and sulfate group (the major components of fucoidan), the purity of the final sulfated polysaccharides product is nearly 84.0%, and this purified fucoidan product was found to display biological activities linked to anti-lipogenesis with 28.9% decreased lipid synthesis in contrast to the free-fatty-acid-induced control.
AB - Fucoidans are sulfated polysaccharides of marine origin and they have distinguished biological applications due to their anti-viral, anti-inflammatory, anti-angiogenic, and anti-tumorigenic potential. In this study, fucoidan was extracted from a brown macroalga (Sargassum siliquosum) using microwave-assisted extraction (MAE) and ultrasound-assisted extraction (UAE). MAE under the condition of 750 W, 10 min, and a liquid/solid ratio of 15 mL/g, showed higher extraction efficiency with a total sugar yield of 6.94% per dry weight. The crude fucoidan obtained with ethanol precipitation was further purified by isoelectric point precipitation and calcium chloride addition (for protein and uronic acid removal), followed by anion-exchange chromatography (AEC) and dialysis. The purity of the polysaccharides content of crude fucoidan increased from 33.3–64.5% with a total sugar recovery of 50.3%. Based on the sugar and sulfate group (the major components of fucoidan), the purity of the final sulfated polysaccharides product is nearly 84.0%, and this purified fucoidan product was found to display biological activities linked to anti-lipogenesis with 28.9% decreased lipid synthesis in contrast to the free-fatty-acid-induced control.
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U2 - 10.1016/j.bej.2020.107798
DO - 10.1016/j.bej.2020.107798
M3 - Article
AN - SCOPUS:85091908382
SN - 1369-703X
VL - 165
JO - Biochemical Engineering Journal
JF - Biochemical Engineering Journal
M1 - 107798
ER -
