Isolation of cellulose-hydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production

Yung Chung Lo; Ganesh D. Saratale; Wen Ming Chen; Ming Der Bai; Jo Shu Chang

doi:10.1016/j.enzmictec.2009.03.002

Isolation of cellulose-hydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production

Yung Chung Lo, Ganesh D. Saratale, Wen Ming Chen, Ming Der Bai, Jo Shu Chang

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

123 Citations (Scopus)

Abstract

Nine cellulolytic bacterial strains were isolated from soil sample taken in southern Taiwan. Through 16S rRNA sequence matching; eight of those isolates belong to Cellulomonas sp., while the other one belongs to Cellulosimicrobium cellulans. The activity of cellulolytic enzymes (cellulases and xylanase) produced from those strains was mainly present extracellularly and the enzyme production was dependent on cellulosic substrates (xylan, rice husk and rice straw) used for growth. HPLC analysis confirmed the bacterial hydrolysis of these cellulosic substrates for soluble sugars production. The efficiency of fermentative H₂ production from the enzymatically hydrolyzed rice husk was examined with seven H₂-producing pure bacterial isolates. With an initial reducing sugar concentration of 0.36 g l^-1, only Clostridium butyricum CGS5 exhibited efficient H₂ production from the rice husk hydrolysates with a cumulative H₂ production and H₂ yield of 88.1 ml l^-1 and 19.15 mmol H₂ (g reducing sugar)^-1 (or 17.24 mmol H₂ (g cellulose)^-1), respectively.

Original language	English
Pages (from-to)	417-425
Number of pages	9
Journal	Enzyme and Microbial Technology
Volume	44
Issue number	6-7
DOIs	https://doi.org/10.1016/j.enzmictec.2009.03.002
Publication status	Published - 2009 Jun 8

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology

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10.1016/j.enzmictec.2009.03.002

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title = "Isolation of cellulose-hydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production",

abstract = "Nine cellulolytic bacterial strains were isolated from soil sample taken in southern Taiwan. Through 16S rRNA sequence matching; eight of those isolates belong to Cellulomonas sp., while the other one belongs to Cellulosimicrobium cellulans. The activity of cellulolytic enzymes (cellulases and xylanase) produced from those strains was mainly present extracellularly and the enzyme production was dependent on cellulosic substrates (xylan, rice husk and rice straw) used for growth. HPLC analysis confirmed the bacterial hydrolysis of these cellulosic substrates for soluble sugars production. The efficiency of fermentative H2 production from the enzymatically hydrolyzed rice husk was examined with seven H2-producing pure bacterial isolates. With an initial reducing sugar concentration of 0.36 g l-1, only Clostridium butyricum CGS5 exhibited efficient H2 production from the rice husk hydrolysates with a cumulative H2 production and H2 yield of 88.1 ml l-1 and 19.15 mmol H2 (g reducing sugar)-1 (or 17.24 mmol H2 (g cellulose)-1), respectively.",

author = "Lo, {Yung Chung} and Saratale, {Ganesh D.} and Chen, {Wen Ming} and Bai, {Ming Der} and Chang, {Jo Shu}",

note = "Funding Information: The authors gratefully acknowledge financial support by Taiwan's Bureau of Energy, Ministry of Economical Affairs. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.enzmictec.2009.03.002",

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AU - Lo, Yung Chung

AU - Saratale, Ganesh D.

AU - Chen, Wen Ming

AU - Bai, Ming Der

AU - Chang, Jo Shu

N1 - Funding Information: The authors gratefully acknowledge financial support by Taiwan's Bureau of Energy, Ministry of Economical Affairs. Copyright: Copyright 2009 Elsevier B.V., All rights reserved.

PY - 2009/6/8

Y1 - 2009/6/8

N2 - Nine cellulolytic bacterial strains were isolated from soil sample taken in southern Taiwan. Through 16S rRNA sequence matching; eight of those isolates belong to Cellulomonas sp., while the other one belongs to Cellulosimicrobium cellulans. The activity of cellulolytic enzymes (cellulases and xylanase) produced from those strains was mainly present extracellularly and the enzyme production was dependent on cellulosic substrates (xylan, rice husk and rice straw) used for growth. HPLC analysis confirmed the bacterial hydrolysis of these cellulosic substrates for soluble sugars production. The efficiency of fermentative H2 production from the enzymatically hydrolyzed rice husk was examined with seven H2-producing pure bacterial isolates. With an initial reducing sugar concentration of 0.36 g l-1, only Clostridium butyricum CGS5 exhibited efficient H2 production from the rice husk hydrolysates with a cumulative H2 production and H2 yield of 88.1 ml l-1 and 19.15 mmol H2 (g reducing sugar)-1 (or 17.24 mmol H2 (g cellulose)-1), respectively.

AB - Nine cellulolytic bacterial strains were isolated from soil sample taken in southern Taiwan. Through 16S rRNA sequence matching; eight of those isolates belong to Cellulomonas sp., while the other one belongs to Cellulosimicrobium cellulans. The activity of cellulolytic enzymes (cellulases and xylanase) produced from those strains was mainly present extracellularly and the enzyme production was dependent on cellulosic substrates (xylan, rice husk and rice straw) used for growth. HPLC analysis confirmed the bacterial hydrolysis of these cellulosic substrates for soluble sugars production. The efficiency of fermentative H2 production from the enzymatically hydrolyzed rice husk was examined with seven H2-producing pure bacterial isolates. With an initial reducing sugar concentration of 0.36 g l-1, only Clostridium butyricum CGS5 exhibited efficient H2 production from the rice husk hydrolysates with a cumulative H2 production and H2 yield of 88.1 ml l-1 and 19.15 mmol H2 (g reducing sugar)-1 (or 17.24 mmol H2 (g cellulose)-1), respectively.

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Isolation of cellulose-hydrolytic bacteria and applications of the cellulolytic enzymes for cellulosic biohydrogen production

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